RP-HPLC METHOD DEVELOPMENT AND VALIDATION FOR ESTIMATION OF CLINDAMYCIN PHOSPHATE AND CLOTRIMAZOLE IN PHARMACEUTICAL DOSAGE FORMS
Journal Title: International Research Journal of Pharmacy (IRJP) - Year 2013, Vol 4, Issue 7
Abstract
A simple, efficient and reproducible Reverse Phase-High Performance Liquid Chromatography (RP-HPLC) method for simultaneous determination of Clindamycin phosphate and Clotrimazole in combined soft gelatin pessaries pharmaceutical formulation has been developed. The separation was carried out on Hypersil BDS C8 (250 × 4.6 mn; 5 µm) column using buffer, 13.6 g Potassium dihydrogen ortho phosphate in 1000 ml of water (adjusted to pH 2.5 ortho phosphoric acid): acetonitrile: l in the ratio of 70:30 v/v as eluent. The flow rate was 1.0 ml / min and effluent was detected at 210 nm. The retention times of Clindamycin phosphate and Clotrimazole were 4.47 minutes, 22.06 minutes and 12.03 minutes respectively. The percentage recovery was within the range between 102.2 % and 103.23 % for Clindamycin phosphate, 98.96 % and 100.54 % for Clotrimazole. The linear ranges were found to be 200 mg / ml (r2 = 0.9998) for Clindamycin phosphate, 400 mg / ml (r2 = 0.9895) for Clotrimazole. The percentage relative standard deviation for accuracy and precision was found to be less than 2 %. The linearity was found to be in the range of 80-120 mg / ml and correlation coefficient of were found to be 0.9998, 0.9979, for Clindamycin Phosphate, Clotrimazole respectively. The proposed method is accurate with 102 % recovery and precise (% RSD of Reproducibility repeatability, intra-day and inter-day variations were 0.19, 0.24, 0.44, 0.78, 0.12-0.15, 0.13-0.37, 0.14, 0.22). The method was successfully applied to pharmaceutical formulation because no chromatographic interferences from peccaries excipients were found.
Authors and Affiliations
R. Rajameena, K. Rama, C. Muthulakshmi
Keywords
Related Articles
- EP ID EP141191
- DOI 10.7897/2230-8407.04731
- Views 68
- Downloads 0
FORMULATION AND DEVELOPMENT STRATEGIES FOR DRUGS INSOLUBLE IN GASTRIC FLUID
A success of formulation depends on how efficiently it makes the drug available at the site of action. Therapeutic effectiveness of a drug depends upon the bioavailability and ultimately upon the solubility of drug molec...
A REVIEW ON: THEVETIA PERUVIANA
Thevetia peruviana are potentially lethal plants after ingestion. The plants is common toxicological emergency in tropical and subtropical parts of the world and international self-harm, using T.peruviana is prevalent i...
EFFECT OF PETROLEUM ETHER EXTRACT OF MALKANGNI (CELESTROUS PENICULATUS WILD) ON OPEN FIELD BEHAVIOR IN SWISS ALBINO MICE
The effects of an acute and repeated administration of petroleum ether extract from the seeds of Malkangani (Celestrus Paniculatus Wild) on explorarity activity and emotional reactivity was examined on open field behavio...
SNAKE BITE MANAGEMENT: AN OVERVIEW
Ayurveda has its own way of approach towards the management of visha, which is unique and is parlance with the concepts of current science. Snakebite remains an underestimated cause of accidental death in India. Estimate...
STUDY ON EXTRACTS OF PARMELIA PERLATA ACH. FOR ITS ANTIMICROBIAL POTENTIAL AGAINST CERTAIN MICROORGANISMS
A significant increase in the trend of infectious diseases and alarming shoot up of resistance to existing antimicrobial agents demands a renewed effort to seek agents effective against pathogenic bacteria resistant to c...