RP-HPLC Method Development and Validation for the Simultaneous Estimation of Etodolac and Paracetamol in Tablet Dosage Form
Journal Title: International Journal of Pharmacy and Analytical Research - Year 2014, Vol 3, Issue 1
Abstract
A simple reversed-phase high-performance liquid chromatographic (RP-HPLC) method has been developed and validated for simultaneous determination of Etodolac and Paracetamol in pharmaceutical tablet dosage form. Chromatographic analysis was performed on a Symmetry X-terra C8 (4.6mm x 150mm,5m) column at ambient temperature with a mixture of mixed ortho phosphoric acid buffer and Acetonitrile in the ratio 50:50 v/v OPA buffer ; as mobile phase, at a flow rate of 0.9 mL min-1. UV detection was performed at 260 nm. The method was validated for accuracy, precision, linearity and sensitivity. The retention times of Etodolac and Paracetamol were 2.190 and 3.373 min, respectively. Calibration plots were linear over the concentration ranges 4–20 μg mL-1 and 5-25μg mL-1 for Etodolac and Paracetamol, respectively. The Limit of detection was 0.361 and 0.559µg mL-1 and the quantification limit were 1.203 µg mL-1 and 1.864µg mL-1 for Etodolac and Paracetamol, respectively. The accuracy of the proposed method was determined by recovery studies and found to be 99.23% to 99.93%. Commercial tablet formulation was successfully analyzed using the developed method and the proposed method is applicable to routine analysis of determination of Etodolac and Paracetamol in pharmaceutical tablet dosage form.
Authors and Affiliations
Tabassum .
Keywords
Related Articles
- EP ID EP656060
- DOI -
- Views 60
- Downloads 0
Chromatographical fractionation guided by antioxidant activity of Morinda citrifolia
Morinda citrifolia Linn. belongs to family of Rubiaceae. Characteristically, the plant is ascribable by its elongated trunk with 8-10 inches long bright green leaves. The leaves are opposite, pinnately veined and glossy....
Spectral analysis of whole plant ethanol extract of Blepharisrepens (vahl) roth and its fraction
The whole plant of Blepharisrepens (vahl) Roth, have a great medicinal value. The aim of the present study was to determine the chemical profile of ethanolic extract of Blepharisrepens (vahl) Rothand its fractions (by va...
Analytical method development and validation for residual solvent of Diltiazem hydrochloride extended release capsule by Gas chromatography
Residual solvents are organic volatile chemicals that are either used or produced during the manufacture of active pharmaceutical ingredients, excipients, and drug products. Organic solvents such as di ethyl ether, N hep...
Spectrophotometric methods for quantitative estimation of sertraline hydrochloride from tablet formulation
Two simple and sensitive visible spectrophotometric methods have been developed for the quantitative estimation of Sertraline Hydrochloride from its tablet formulation. The developed methods are based on formation of chl...
Fabrication and evaluation of a stable flurbiprofen hydrogel
The purpose of the study was to prepare a stable 6% Flurbiprofen hydrogel. Oral Flurbiprofen generates several side effects; hence hydrogel was prepared to reduce these side effects in the present study. Gastrointestinal...