RP-HPLC METHOD DEVELOPMENT FOR THE SUBSTITUTED BENZENE DERIVATIVE OF EUGENOL
Journal Title: International Research Journal of Pharmacy (IRJP) - Year 2013, Vol 4, Issue 11
Abstract
A simple Reverse phase liquid chromatographic method has been developed and subsequently validated for the derivative of the Phenyl substituted derivatives on the hydroxyl proton of Eugenol. The separation was carried out using a mobile phase consisting of 0.5 %v/v Acetic Acid buffer in HPLC grade water (Mobile phase A) and Methanol (Mobile phase B). The column Inertsil ODS, 3V (C18, 5 μ, 25 cm × 4.6 mm id with flow rate of 1.0 ml / minute using PDA detection at 260 nm. The described method was linear over a concentration range of 10-100 μg/ml (ppm). All the five mixtures are well separated and the resolution complies as per the pharmacopeia method development requirements. The results of the study showed that the proposed RP-HPLC method is simple, rapid, precise and accurate, which is useful for the identification and quantifications of the said derivatives of 4-allyl-2-methoxyphenyl 3,4-dimethoxybenzoate (RLS-228), 4-allyl-2-methoxyphenyl 3,4,5-trimethoxybenzoate (RLS-222), (Z)-4-allyl-2-methoxyphenyl 3-(4-methoxyphenyl) acrylate (RLS-205), 4-allyl-2-methoxyphenyl 4-ethoxy-3-methoxybenzoate (RLS-193) and 4- allyl-2-methoxyphenyl benzoate (RLS-218)
Authors and Affiliations
Ayyavoo Kaliyan, Vijay Gangan, Trupti Bamne
Keywords
Related Articles
- EP ID EP141518
- DOI 10.7897/2230-8407.041124
- Views 111
- Downloads 0
NEW HORIZON ON ETIOPATHOGENESIS OF AIDS
AIDS, the dread full disease of this century is mainly due to abnormal sexuality. Epidemiological studies state that five to ten million people are infected with HIV among which 61% cases are due to abnormal sexuality. A...
CRONOBACTER SAKAZAKII: AN EMERGING CONTAMINANT IN PEDIATRIC INFANT MILK FORMULA
Cronobacter sakazakii (C. sakazakii) previously known as Enterobacter sakazakii is a motile, Gram-negative, non-sporing yellow pigmented rod, which belongs to lethal Enterobacteriaceae family. C. sakazakii is ubiquitousl...
A REVIEW ON PHARMACEUTICAL PROCESS VALIDATION
Validation is one of the important steps in achieving and maintaining the quality of the final product. Validation of the individual steps of the process is called process validation. The process is developed in such a w...
THE LEAVE EXTRACTS OF HEERA INSIGNIS (DEL) INHIBITS RESISTANCE BACTERIA, FUNGI AND MYCOBACTERIUM BOVIS
The hexane (HE), dichloromethane (DCM), ethylacetate (EA) and methanol (ME) extracts of the leaves of Heeria insignis. A plant with wide ethnomedicinal application in Northern Nigeria, was evaluated for anti-tuberculosis...
DEVELOPMENT AND IN VITRO EVALUATION OF MOUTH DISSOLVING FILMS OF CAPTOPRIL
Captopril is a potent, competitive inhibitor of angiotensin-converting enzyme(ACE), the enzyme responsible for the conversion of angiotensin I (AT I) to angiotensinII (AT II). Captoprilmaybeusedinthetreatmentofhypertensi...