RP- HPLC METHOD FOR SIMULTANEOUS ESTIMATION OF MONTELUKAST SODIUMAND DESLORATIDINE FROM BULK AND TABLETS FORMULATION
Journal Title: International Research Journal of Pharmacy (IRJP) - Year 2012, Vol 3, Issue 7
Abstract
A simple, rapid, accurate, precise and validated High performance Liquid Chromatographic method for the simultaneous estimation of Desloratidine(DES) and Montelukast sodium(MON) in bulk and Multicomponant formulation. The Reversed-phase liquid chromatographic analysis was performed on a Hypersil C18 column (250mm, 4.6mm i.d., 5 μm particle size) column with mobile phase Acetonitrile: 0.05M Potassium Dihydrogen ortho-phosphates with 1.5ml Triethylamine of pH 3 (65: 35 v/v) and column temperature at 40°C. The flow rate of the mobile phase was adjusted to 1.3 mL/min and the injection volume was 10 μl. Detection was performed at 210 nm. The retention time for DES and MON were 2.12 min and 12.76 min respectively. The method was validated and shown to be linear for DES and MON in 10-60 µg/mL (r2=0.998) and 20-120 µg/mL (r2=0.998) respectively. The relative standard deviation of DES and MON for intra-day was 0.2746 and 0.7637 respectively, inter-day was found to be, 0.7836 and 0.9317 respectively. The developed RP- HPLC method is suitable for estimation of Montelukast sodium and Desloratidine in tablet formulation. Hence this method can be used in quality control for routine analysis of the finish drug product.
Authors and Affiliations
Tukaram Kalyankar , Ranjeet Kokate , Rajendra Kakade
Keywords
Related Articles
- EP ID EP135259
- DOI -
- Views 94
- Downloads 0
FORMULATION AND EVALUATION OF TASTE MASKED ORALLY DISINTEGRATING TABLETS OF SITAGLIPTIN PHOSPHATE MONOHYDRATE
The purpose of the work is to mask the unpleasant taste of sitagliptin phosphate monohydrate with mannitol by co-grinding method and to formulate it as an oral disintegrating tablet by direct compression method. Drug-man...
DEVELOPMENT AND VALIDATION OF A GREEN BIOANALYTICAL METHOD FOR THE DETERMINATION OF SPARFLOXACIN IN PHARMACEUTICAL DOSAGE FORM AND HUMAN PLASMA BY RP-HPLC
This study describes the development of an innovative, green, rapid, precise, selective and sensitive reverse phase high-performance liquid chromatography method for the quantitative determination of Sparfloxacin (SPR) i...
EVALUATION OF NOOTROPIC ACTIVITY OF POLYHERBAL FORMULATION SR-105 IN EXPERIMENTAL ANIMALS
The main objective of the proposed work is to evaluate the beneficial effect of SR-105 on CNS mainly for its locomotor and nootropic activities in different experimental animal models like passive paradigm, sodium nitrit...
PHYTOCHEMICAL SCREENING OF GARHWAL HIMALAYA WILD EDIBLE TUBER COLOCASIA ESCULENTA
The present study aimed at evaluating the nutritional profile, successive value, thin layer chromatography and phytochemical screening of Colocasia esculenta. It is an important medicinal plant in India which is used in...
ISOCRATIC SEPARATION OF FOUR BETA BLOCKERS WITH AMLODIPINE BY C18 RP-HPLC: APPLICATION TO AMLODIPINE DETERMINATION IN PHARMACEUTICAL DOSAGE FORMS
This method described for the successful separation of a beta blockers with amlodipine by RP-HPLC on a C18 column with UV detection. One of the key goals of High Performance Liquid Chromatography technique is to achieve...