Salt Tolerance Evaluation in Cotton (Gossypium hirsutum L.) Using RAPD Marker
Journal Title: Журнал ÑтреÑÑ-физиологии и биохимии - Year 2015, Vol 11, Issue 4
Abstract
The aim of this study was to evaluate four upland cotton ([i]Gossypium hirsutum[/i] L.) [Niab78 (N78), Deir-Ezzor22 (DE22), Deltapine50 (DP50) and Aleppo118 (A118)] varieties towards salt stress (0 and 200 mM NaCl) for 7 weeks based on RAPD marker. Our data showed that the highest total polymorphic bands identified by the 26 RAPD tested primers were 150 bands generated by N78 variety, while the lowest ones were recorded for DP50 (29 bands). Otherwise, unique (negative and positive) markers characterized the two tolerant varieties (N78 and DE22) were 22 and 29 markers, respectively. Our data indicated that the highest polymorphism level was detected in N78 variety (68.5%) followed by DE22 (60.9%), whereas, the lowest one was recorded for DP50 (21.3%). Our data obtained herein indicates that RAPD marker provided molecular markers for salinity tolerance screening in early stage in genetic improvement programs.
Authors and Affiliations
Basel Saleh
Keywords
Related Articles
- EP ID EP133140
- DOI -
- Views 113
- Downloads 0
Physiological Responses, Growth Rate and Blood Metabolites Under Feed Restriction and Thermal Exposure in Kids
The study was carried out to study the cumulative effect of thermal stress and feed restriction in kids. Twelve kids of Alpine x Beetle cross were divided into two groups. Group 1 served as control and group 2 was put on...
Изменение содержания оксида азота в корнях проростков пшеницы под влиянием лектинов азоспирилл
Показано, что лектин Azospirillum brasilense Sp7 в концентрации 40 мкг/мл вызывает два пика индукции синтеза оксида азота в корнях проростков пшеницы, происходящей через 3 и 26 ч совместной инкубации. Лектин мутантного п...
Determination of Lake Baikal endemic amphipod Eulimnogammarus verrucosus (Gerstf., 1858) thermal optima limits by changes in its metaboliс markers
In present study the determination of cellular metabolic markers under exposure of Lake Baikal endemic amphipod Eulimnogammarus verrucosus (Amphipoda, Crustacea) in a wide range of ambient temperatures was carried out. T...
Application of DNA (RAPD) and ultrastructure to detect the effect of cadmium stress in Egyptian clover and Sudan grass plantlets
Background In recent years, several plant species have been used as bioindicators to evaluate the toxicity of environmental contaminants on vegetal organisms. In this study, Egyptian clover and Sudan grass seedlings wer...
FUNCTIONAL SPECIALIZATION OF DUPLICATED FLAVONOID BIOSYNTHESIS GENES IN WHEAT