Screening and genotyping of Mur blood group among voluntary blood donors in the population of Hezhou, Guangxi
Journal Title: Chinese Journal of Blood Transfusion - Year 2024, Vol 37, Issue 7
Abstract
Objective To screen the distribution frequency of Mur blood group among voluntary blood donors in Hezhou, Guangxi, and further analyze the molecular basis of of Mur antigen positive samples. Methods The Mur phenotype of voluntary blood donors in Hezhou was serologically screened using microplate method, and the distribution frequency of Mur antigens in different ethnic groups was analyzed. Genetic typing was performed on these positive samples with PCR-SSP method to verify the accuracy of the serological method, and the genetic background was sequenced and analyzed. Results Among 3 298 samples from voluntary blood donors in Hezhou, 432(13.10%, 432/3 298) were screened positive for Mur antigen, and PCR-SSP genotyping validation showed that all 432 samples were electrophoretic positive. Among them, the proportion of Han blood donors with positive Mur antigen was 12.79%(331/2 587), Yao ethnic group was 13.25%(64/483), Zhuang ethnic group was 16.51%(36/218), and no statistically significant difference was found in the three groups(P>0.05). Further sequencing results showed that 428 samples were GYP(B-A-B) Mur, also known as GYP. Mur type(12.98%, 428/3 298), the other 4 samples were GYP(B-A-B) Bun, also known as GYP. Bun type(0.12%, 4/3 298). Conclusion The Mur blood type frequency is high in the voluntary blood donors in Hezhou, Guangxi, and is predominant characterized by GYP. Mur genotype. Due to ethnic integration, no significant difference was noticed in the frequency of Mur blood type distribution between Han, Zhuang and Yao population. Therefore, conducting extensive Mur blood group antigen and antibody testing in Hezhou is of great significance for ensuring clinical blood transfusion safety.
Authors and Affiliations
Weiquan YUAN, Shaohua DING, Jianmin LI, Xueming WU, Shengming WEN, Houquan LIN, Weisheng HE, Xiaoming LI, Jiajie ZHANG, Longming XIAO, Shengbao DUAN, Shengwang CHEN
Keywords
Related Articles
- EP ID EP742812
- DOI 10.13303/j.cjbt.issn.1004-549x.2024.07.009
- Views 43
- Downloads 0
Study on the extensibility of platelet donor gene database in Shaanxi
Objective To analyze the commonality and characteristics between voluntary blood donors and hematopoietic stem cell donors in this region, and explore the potential for integration and development between China Marrow Do...
Multiple antibodies against -E, -c, -M, and -S: a case report
Objective To identify antibody specificity in an elderly patient with hydronephrosis accompanied by ureteral stones and shock who had multiple antibodies. Methods Microcolumn gel method was used to screen unexpected anti...
Analysis of chylemia and its impact on blood product quality
Objective To analyze the causes of chylemia, explore the effects of varying degrees of chylemia on subsequent blood products and the countermeasures. Methods The information of blood donors in the Central Blood Station o...
Analysis on the volume control of red blood cells in additive solution produced by Chongqing blood services
Objective To investigate the current situation concerning volume control of red blood cells in additive solution produced by blood service in Chongqing, and to lay a foundation for promoting the homogenization of prepara...
Severe Streptococcus pneumoniae-associated red blood cell T-polyagglutination and plasma exchange strategy
Objective To identify and propose blood transfusion suggestions for 3 children suspected to have red blood cell T polyagglutination. Methods According to the RBC reactions with phytohemagglutinin, adult serum and cord bl...