Screening and Isolation of Keratinase Producing Micro Organisms
Journal Title: International Journal for Research in Applied Science and Engineering Technology (IJRASET) - Year 2017, Vol 5, Issue 5
Abstract
In present study 35 proteolytic isolates were isolated from various samples containing keratin rich sources from different areas in Kolhapur and Sangli region of Maharashtra state. These isolates were screened for production of proteolytic enzymes like caseinase,gelatinase by using milk agar and gelatin agar. All these isolates were gelatinase and caseinase positive.So, further used to check kertinolytic activity by studying feather degradation process.From all isolates,7 isolates named as I1, I6, P, M5-2,E2N,E4N,C9 shows keratinase activity.Out of which,isolate M5-2 show highest caseinase(10 mm zone),gelatinase(26 mm zone) activity along with 100% feather degradation(keratinase) activity.This keratinolytic isolate was further identified morphologically,biochemically and by 16s rRNA sequencing as Stenotrophomonas maltophilia YArck. The sequence is deposited at Gen Bank NCBI with gene accession no.KY941138.
Authors and Affiliations
Aishwarya B. Kurane, Yasmin. C. Attar
Keywords
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