Screening of Agrobacterium Mediated Transient Transformation of Peanut (Arachis hypogaea)
Journal Title: Journal of Plant Science and Research - Year 2017, Vol 4, Issue 2
Abstract
Peanut is a cash crop having outstanding food values. Therefore, it can be used as a supplementary food for hunger and malnutrition affecting Asian countries like Bangladesh. But peanut yield is substantially reduced for abiotic and biotic stresses. Novel and beneficial genes into peanut need to be introduced through genetic manipulation that would not be available using conventional breeding methods. Thus, Agrobacterium mediated genetic transformation may lead the solution. Present study was examined with two Bangladeshi peanut varieties, such as, BINA Chinabadam 4 and BINA Chinabadam 6 where decapitated half embryo was used as explant for transformation. Agrobacterium tumefaciens strain LBA4404 with plasmid constructs, pBI121 was used for infection in the transformation experiment. The total size of pBI121 is 12.8 kb according to its construction map. The T-DNA of Ti plasmid contains a plant selectable marker gene neomycin phosphotransferase II (npt II) conferring resistance to kanamycin and a uidA gene encoding β-gluduronidase (GUS) reporter gene (1812 bp). Explants having different transformation treatments (different OD600, infection and co-cultivation duration, age of the embryo) were taken for GUS histochemical assay. Transformation study was conducted with 1 day old decapitated half embryo where highest percentage of GUS positive explants were found when OD600 was more than 1 at 60 minutes of infection and 3 days of co-cultivation duration in transient assay.
Authors and Affiliations
Kashmery Khan
Keywords
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