Symbiotic N Fixation: Nodulation of Tepary Bean (Phaseolus acutifolius A. Gray)
Journal Title: Journal of Plant Science and Research - Year 2016, Vol 3, Issue 1
Abstract
Tepary Bean (Phaseolus acutifolius A. Gray), is known to be a drought-tolerant grain legume and could play a positive role in supporting sustainable role in production of food and feed under changing climatic conditions due to its’ ability to Symbiotically fix atmospheric N. However, existence of any rhizobial strain that could nodulate Tepary bean was unknown before the initiation of this study. We evaluated nodulation in 51 Tepary bean accessions after inoculation with a rhizobial strain (Phaseolus Spec #3) from Novozymes BioAg, Inc. to determine existence, if present, of nodulation. Our results indicated that Tepary bean could be successfully nodulated by using Phaseolus Spec #3 strain. We grew 51 Tepary bean accessions in a mixture of sand and vermiculite in two experiments and inoculated seeds with Phaseolus Spec #3 and recorded data on nodule size scores (1= small sized nodules similar in size to mustard seed; 2= medium sized nodules; varying from mustard seed size to soybean seed size and 3= large sized nodules greater in size than soybean seed), nodule number scores (1= less than 5 nodules; 2= more than 10 nodules; and 3= more than 20 nodules). A SPAD (SPAD-502, Minolta Corporation) meter was used to record relative chlorophyll in each plant prior to harvesting. A new variable named “Combined Score” was computed by summing values for nodule size scores, nodule number scores, and SPAD readings. The SPAD unit reading, nodule size score, nodule number score, and combined score means were 27.08, 1.48, 2.12, and 30.68, respectively. SPAD reading exhibited 0.44 and 0.61 correlation coefficient (significant at 1% level), respectively with nodule size score and module number score. Based on results of this study, we conclude that Tepary bean can be nodulated by Phaseolus Spec #3 rhizobial strain.
Authors and Affiliations
Harbans L Bhardwaj
Keywords
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