SCREENING OF CHITINASE PRODUCTION AND ANTIFUNGAL ACTIVITY OF STREPTOMYCES SP. ACT7 FROM EAST COAST REGION, SOUTH INDIA
Journal Title: International Journal of Pharmacy and Pharmaceutical Sciences - Year 2015, Vol 7, Issue 5
Abstract
Objective: The objective of the present study was aimed to isolate actinobacteria from the marine environment and to screen the production and antifungal activity of chitinase enzyme against phytopathogenic fungi.Methods: The actinobacteria were isolated from twelve sediment samples of East Coast Regions (ECR) by spread plate technique on starch casein agar, and the actinobacteria isolates were identified by the methods of International Streptomyces Project (ISP). All the actinobacteria were tested for chitinase activities using enrichment chitin agar media (EMCA) by spot inoculation method, and the potential isolate was used for chitinase production by submerged fermentation technique using 1% colloidal chitin in liquid medium. The efficacy of chitinase activity was assessed by spectrophotometric method along with growth monitoring. The most potential isolate Streptomyces sp. ACT7 was evaluated for its antagonistic activity against phytopathogens namely F. oxysporum and Alternaria sp. by dual culture method.Results: Totally, 27 isolates of actinobacteria were isolated from marine sediments of ECR. Of them, 9 isolates had chitinase activity. Among the chitinase producers, the isolate ACT7 had prominent chitinase production after 7 days of incubation. The antagonistic activity of the isolate ACT7 was effective in restricting the radial growth of phytopathogens such as F. oxysporum and Alternaria sp.Conclusion: The actinobacteria has found to possess potent antifungal activity and it can be used as a promising biocontrol agent, thereby reducing the use of chemical fungicides.Â
Authors and Affiliations
D. Thirumurugan, R. Vijayakumar
Keywords
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