Screening of Mentha cordifolia Opiz (Yerba Buena) buffer crude extract for aspartyl protease pepsin inhibitory activity
Journal Title: International Journal of Research in Pharmacology & Pharmacotherapeutics (IJRPP) - Year 2014, Vol 3, Issue 1
Abstract
To date, several hundred bioactive compounds have been isolated from plant sources. Among them, protease inhibitors have drawn the attention recently owing to their pivotal role in pharmaceutical industry. With the anticipation that commonly and widely available plant could provide pharmacologically important protease inhibitor, an attempt will be made to screen Mentha cordifolia Opiz (Yerba buena) for aspartyl protease pepsin inhibitor to select a potential candidate for possible HIV-1 protease inhibitor. This research is an experimental type of study. It specifies the inhibitory activity of Mentha cordifolia Opiz (Yerba buena) against aspartyl protease pepsin. Plant extracts preparation, pepsin kinetic assay, pepsin inhibitory activity assay, qualitative phytochemical screening and Total Flavonoid Content were employed in the methodology. The percent inhibition of various concentration of Pepstatin A 12.5, 25, 50, 100 and 1,000ug/ml obtained 63.43%, 72.26%, 78.62%, 82.86% and 96.47% respectively. All negative control concentrations were above 60% inhibitory activity. Mentha cordifolia Opiz lyophilized buffer crude extract concentrations 12.5, 25, 50, 100 and 1,000ug/mL obtained 57.95%, 62.46%, 69.32%, 74.37% and 94.96% respectively with an IC60 value of 17.30ug/mL. The study aims to determine the inhibitory activity of Mentha cordifolia Opiz (Yerba buena) on aspartyl protease pepsin. Relating to the objectives of the study and based on the results of the experimentations, the proponent now concludes: Mentha codifolia Opiz (Yerba buena) have Concentration 60% (IC60) value of 17.30ug/mL against pepsin and the effect of lyophilized buffer crude extract of Mentha codifolia Opiz (Yerba buena) shows increasing response as the concentration of the plant lyophilized buffer extract increases.
Authors and Affiliations
Alfredo A. Hinay Jr
Keywords
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