Seed storage protein electrophoresis for identification of some Groundnut (Arachis hypogaea L.) cultivars
Journal Title: International Research Journal of Applied and Basic Sciences - Year 2015, Vol 9, Issue 10
Abstract
To survey of genetic relation and diversity among peanut genotypes, 11 cultivars of peanut were planted in the year 2013 at a research farm in Lasht-e Nesha in randomized complete block design with three replications. Protein bands were analyzed with the Total lab software and results showed that the 17 visible major bands were showed on the vertical gel. Increase of expression levels in a specific protein (band No.11 approximately 26 kDa weight) was expressed in all cultivars. Cluster analysis with UPGMA method on the basis of protein bands and cut the dendrogam at 50% similarity, divided the cultivars in 4 groups. According to the results, Genotypes 7, 8, 9, 10 and 11 in the first group, genotype 3, 4 and 5 in the second groups and Genotypes 1 and 2, were in the third and fourth groups separately. In general, the results of this study showed that the seed storage proteins of peanut cultivars were suitable for evaluation of genetic diversity and dividing the cultivars in separately groups.
Authors and Affiliations
Jamalomidi Masoomeh *| Department of Biology, Payame Noor University, PO BOX 19395-3697 Tehran, Iran, email: mjamalomidi@pnu.ac.ir, Gholami Mehran| Department of seed and plant improvement, Guilan Agricultural and Natural Resource Research and Education Center, Rasht, Iran, Jamalomidi Fatemeh| Department of Biology, Faculty of Science, University of Guilan, Rasht, Iran
Keywords
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