Selection of Suitable Microorganism for Biocatalytic Oxidation Reaction of Racemic Propranolol
Journal Title: Gazi Üniversitesi Fen Bilimleri Dergisi Part C: Tasarım ve Teknoloji - Year 2017, Vol 5, Issue 4
Abstract
Propranolol is one of the β-blockers which are pharmaceutically important, especially used for treatment of cardiovasculer disease. In this study, the production of enantiomerically pure propranolol was aimed via biocatalytic deracemization including tandem oxidation-reduction reactions of racemic propranolol. Within this content, firstly suitable microorganism for the oxidation of racemic propranolol was investigated. Alcohol dehydrogenase (ADH) enzyme for oxidation of propranolol and NADH oxidase enzyme for cofactor regeneration were necessary for the oxidation reactions. For this reason, ADH and NADH oxidase enzymes activities of different microorganisms were measured to select the microorganism for using as enzyme source. These microorganisms are Lactobacillus kefir NRRL B-1839, Rhodotorula glutunis DSM 70398, Rhizopus oryzae CBS 111718, Rhizopus arrhizus. The highest ADH and NADH oxidase activities were obtained for L. kefir.
Authors and Affiliations
Rahime SONGÜR, Ülkü MEHMETOĞLU
Keywords
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- EP ID EP485556
- DOI 10.29109/http-gujsc-gazi-edu-tr.369216
- Views 108
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