Abstract

Abstrac Tacrolimus is used as an immunosuppressant. The method developed is used for assay tacrolimus in pharmaceutical dosage form. A simple, rapid and reproducible high performance reverse phase liquid chromatographic method has been developed for quantitative estimation of Tacrolimus in tablets form using a, Phenomenex 250 mm x 4.9 mm C18 , 5 um, inertsil and UV detection at 220nm. The isocratic elution was used to quantify the analyte and the mobile phase was Acetonitrile: water (50: 50) was pumped at 1.0 ml/min. The method was linear between 50-300 μg/ml, statistically validated for its linearity, precision and accuracy. The intra-and - inter day variation was found to be less than 1% showing high precision of the assay method. The method selective and sensitive which is used for the estimation of tacrolimus in presence of excipients in the commercial capsules did not interfere with the method.

Authors and Affiliations