Seminal plasma proteins increase the post-thaw sperm viability of Sanmartinero bull’s semen
Journal Title: Revista MVZ Cordoba - Year 2013, Vol 18, Issue 1
Abstract
Objectives. This study was performed to evaluate the effect of the addition of proteins on the postthawing viability of spermatozoa. Materials and methods. Spermatozoa were frozen with two different media: Citrate-fructose and Bioxcell®. The isolation of seminal plasma proteins of low molecular weight was performed through low pressure liquid chromatography. It was determined that the proteins of interest eluted in fractions 21-25, and two dimensional electrophoresis was performed. Thawed sperm was incubated at 37°C for one hour with 0.5, 1, 1.5 and 2.0 mg of 21-25 fraction protein. Two additional treatments were included: one with seminal plasma total protein, and another one without protein. Results. Two dimensional electrophoresis of protein confirmed the presence of two bands of 14 and 16 kDa and seven spots with iso-electric points between 5.0 - 5.5 respectively. Incubation of the spermatozoa with the 21-25 fraction showed that sperm viability increases by 20% with doses of 1 and 1.5 mg of protein/106 spermatozoa in the citrate-fructose medium, and 25% with 0.5 mg of protein/106 spermatozoa in Bioxcell® medium. A positive effect in sperm viability was demonstrated although it depends on the doses of protein and the cryopreservation medium used. Conclusions. This investigation suggests that the use of seminal plasma proteins can be useful for reducing the harmful effect on sperm cryopreservation.
Authors and Affiliations
Fabián Rueda A, Lic Quím| Corporación Colombiana de Investigación Agropecuaria CORPOICA. Centro de Biotecnología y Bioindustria. Laboratorio de Microbiología Molecular. Cundinamarca, Colombia., Tatiana Garcés P, Biól| Universidad de Pamplona. Laboratorio del Grupo de Investigación en Química. Ciudad universitaria. Pamplona, Norte de Santander, Colombia., Rocío Herrera L, Zootec| Corporación Colombiana de Investigación Agropecuaria CORPOICA. Centro de Biotecnología y Bioindustria. Laboratorio de Microbiología Molecular. Cundinamarca, Colombia., Luis Arbeláez R, Ph. D| Universidad de Pamplona. Laboratorio del Grupo de Investigación en Química. Ciudad universitaria. Pamplona, Norte de Santander, Colombia., Miguel Peña J, MVZ| CORPOICA. Centro de Reproducción Integral Animal (CRIA). Centro de Investigación La Libertad. Km 21 vía Pto. López, Villavicencio, Colombia., Henry Velásquez P, M. Sc| CORPOICA. Centro de Reproducción Integral Animal (CRIA). Centro de Investigación La Libertad. Km 21 vía Pto. López, Villavicencio, Colombia., Aureliano Hernández V, Ph. D| Universidad Nacional de Colombia. Facultad de Medicina Veterinaria y de Zootecnia. Bogotá, Colombia., Jaime Cardozo C, Ph. D *| Corporación Colombiana de Investigación Agropecuaria CORPOICA. Centro de Biotecnología y Bioindustria. Laboratorio de Microbiología Molecular. Cundinamarca, Colombia.Correspondencia: jcardozo@corpoica.org.co
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