Sensitivity of watermelon variety Bojura to m[sup][/sup]utant agents [sup]60[/sup]Co and EMS[sup][/sup]
Journal Title: Journal of BioScience and Biotechnology - Year 2016, Vol 5, Issue 1
Abstract
A study on the sensitivity of watermelon variety Bojura to mutagenic agents was carried out in 2013-2014. The goal was to establish effective doses for mutagenic treatment of dry seeds with [sup]60[/sup]Co gamma rays (80, 100, 200, 250, 350 and 450 Gy) and swollen seeds with water for 24 hours were treated with ethyl methanesulfonate (EMS) at a concentration of 2%. Dominant mutations were not observed in the M[sub]1[/sub] generation. Morphological changes in 14 of 1395 M[sub]2[/sub] plants were observed. Phenotypic variations changes were the colour of the seed coat, chlorophyll disorders of cotyledons, leaves, petals, and alterations of the location of the fruit set in the central stem. Visible changes of the morphological characteristics of the fruit were not observed. The doses induced certain morphological changes, however, higher doses or combined gamma rays [sup]60[/sup]Co and EMS treatments would induce mutations more efficiently. Subsequent experiments are required to obtain mutants with changes that affect flowers and fruits. The results are important for increasing mutation efficiency in watermelon breeding.
Authors and Affiliations
Nikolay Velkov, Nasya Tomlekova, Fatma Sarsu
Keywords
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