SEPARATION AND DETERMINATION OF PROCESS-RELATED IMPURITIES OF CLOPIDOGREL BISULPHATE BY RP-HPLC
Journal Title: World Journal of Pharmaceutical and life sciences - Year 2017, Vol 3, Issue 1
Abstract
Clopidogrel bisulfate is a potent oral antiplatelet agent. Pro-drug which belongs to the chemical family of Thienopyridine adenosine diphosphate(ADP)-receptor antagonists.[1-2] It is mainly used to reduce atherosclerotic events like myocardial infarction, stroke and vascular death in patients who had a recent stroke, myocardial infarction or have established peripheral vascular disease. The main objective of the present study is to separate Clopidogrel bisulphate and its process related impurities in the drug substance. In order to develop a suitable and robust RP-HPLC method for the determination of Clopidogrel bisulphate and its process related impurities, an attempt was made with a C18 column using mobile phase composed of acetonitrile and water in the ratio 50:50% v/v. Experiments were conducted by using different columns, different buffers and different concentrations of organic modifier in order to optimize the chromatographic conditions. Reasonable separation between all compounds was observed in case of Clopidogrel bisulphate. It was felt necessary to carry out further optimization studies and hence attempts were made in this direction. Finally, a mobile phase composed of potassium dihydrogen phosphate buffer (pH 3.5) and acetonitrile in the ratio 78:22% v/v with a flow rate of 1.0 ml/min gave sharp peaks with minimum tailing and good resolution for both the drug and its impurities.
Authors and Affiliations
Rajkumar Prava
Keywords
Related Articles
- EP ID EP648164
- DOI -
- Views 216
- Downloads 0
BIOCHEMICAL STUDIES OF THE WHEAT (TRITICUM AESTIVUM L)
Tricin (5,7,4'-trihyroxy-3',5'-dimethoxyflavone), a naturally occurring compound, is a characteristic constituent of the grass family, including cereal grain plants, and has been isolated from wheat. Apart from being a p...
ROLE OF ERANDADI TAILA IN THE MANAGEMENT OF KARNA NADA-A CONCEPTUAL STUDY
Karna Nada, a hearing disorder mentioned in Sushruta Samhita can be co-related with tinnitus on the basis of signs and symptoms. As per the literature, Karna Nada is Vata Dosha predominant disorder along with Avarana of...
RESPONSE OF GROWTH AND YIELD OF SHALLOTS (ALLIUM ASCOLANICUM L.) BY POTASSIUM NITRATE (KNO3) AND ORGANIC LIQUID FERTILIZATION
The shallots are one of the leading vegetable commodities, but have not shown a real increase in production every year so that efforts are needed to increase the production of shallots. This study aimed to obtain the opt...
HERBAL MEDICINE: A BOON TO PERIODONTICS A REVIEW
The knowledge on medicinal plants has been based on different medicinal systems such as Ayurveda, Unani and Siddha. In India, plant species serve as regular sources of medicine. Herbal products are preferred due to wide...
IN-VITRO AND IN-VIVO SCREENING METHODS FOR ELUCIDATING THROMBOLYTIC ACTIVITY OF BAUHINIA RACEMOSA (L)
The plant Bauhinia racemosa (L). belongs to the Caesalpiniaceae family. It is popularly known as “Apta” in Marathi, “Kanchnal” in Hindi. Other common names include Mountain Ebony and Kachnar (India and Pakistan). The bar...