SEPARATION AND DETERMINATION OF PROCESS-RELATED IMPURITIES OF CLOPIDOGREL BISULPHATE BY RP-HPLC
Journal Title: World Journal of Pharmaceutical and life sciences - Year 2017, Vol 3, Issue 1
Abstract
Clopidogrel bisulfate is a potent oral antiplatelet agent. Pro-drug which belongs to the chemical family of Thienopyridine adenosine diphosphate(ADP)-receptor antagonists.[1-2] It is mainly used to reduce atherosclerotic events like myocardial infarction, stroke and vascular death in patients who had a recent stroke, myocardial infarction or have established peripheral vascular disease. The main objective of the present study is to separate Clopidogrel bisulphate and its process related impurities in the drug substance. In order to develop a suitable and robust RP-HPLC method for the determination of Clopidogrel bisulphate and its process related impurities, an attempt was made with a C18 column using mobile phase composed of acetonitrile and water in the ratio 50:50% v/v. Experiments were conducted by using different columns, different buffers and different concentrations of organic modifier in order to optimize the chromatographic conditions. Reasonable separation between all compounds was observed in case of Clopidogrel bisulphate. It was felt necessary to carry out further optimization studies and hence attempts were made in this direction. Finally, a mobile phase composed of potassium dihydrogen phosphate buffer (pH 3.5) and acetonitrile in the ratio 78:22% v/v with a flow rate of 1.0 ml/min gave sharp peaks with minimum tailing and good resolution for both the drug and its impurities.
Authors and Affiliations
Rajkumar Prava
Keywords
Related Articles
- EP ID EP648164
- DOI -
- Views 208
- Downloads 0
ARUM CYRENIACUM: INVESTIGATION OF VOLATILES AND HEAVY METALS REMOVING CAPACITY
Arum cyreniacum is an endemic Libyan plant growing in Aljabal Al-Akhder region.Though Arum species have been used since antiquity in herbal medicine for a wide array of human disorders, information on the medicinally imp...
AN ARTICLE ON COLON TARGETED DRUG DELIVERY SYSTEM
Day by day there are new developments in field of colon specific drug delivery system. Colonic drug delivery has gained increased importance not just for the delivery of the drugs for the treatment of local diseases asso...
ROLE OF VAMANA KARMA AND NIMBADI CHURNA WITH PATOLADI KWATHA AS ANUPANA IN THE MANAGEMENT OF EKA KUSHTA W. S. R. PLAQUE PSORIASIS - A REVIEW ARTICLE
In today‟s world everyone sees what you appear to be. Appearance is nothing but the skin. Skin diseases are affecting the mankind since time immortal. Skin is a mirror that reflects external and internal pathology, and i...
A REVIEW: LEAD TOXICITY AND IT’S MEDICOLEGAL ASPECTS
Lead is a metallic inorganic irritant poison. According to Health and Human Services Department USA, lead poisoning is the most important environmental problem. Poisoning is more common from chronic occupational exposure...
USE OF WATERLEAF (TALINUM TRIANGULARE) IN REMEDIATION OF SOIL EXPOSED TO HEAVY METALS: A GREEN TECHNOLOGY APPROACH
The major environmental concern due to the disposal of urban and industrial waste generated by anthropogenic activities is the contamination of soil with heavy metals. The potential of waterleaf (Talinum triangulare) to...