Sequence Analysis of New Isolated O-methyltransferase Transcript (VMPOMT) from Vanda Mimi Palmer
Journal Title: Scholars Academic Journal of Biosciences - Year 2015, Vol 3, Issue 3
Abstract
Abstract: Vanda Mimi Palmer is an orchid hybrid between Vanda Tan Chay Yan and Vanda tessellata. This orchid has won several international awards for its fragrance emission characteristic. To date, scientific studies on enzymes as well as their related genes and transcripts that are involved in floral scent production and biosynthesis are barely covered in fragrant orchids especially in vandaceous orchids. The aims of this study were to isolate full open reading frame (ORF) of O-methyltransferase transcript from Vanda Mimi Palmer (VMPOMT) and followed by sequence characterisation using bioinformatics tools. The size of successfully isolated full-length transcript of VMPOMT (Genbank accession no: KF278721) is 1,289bp, encoding for a polypeptide of 368 amino acid residues consisting of 1,104bp open reading frame (ORF) flanked by 2bp of 5'-untranslated region (UTR) and 182bp of 3'-UTR including a poly-A tail. Sequence analyses using bioinformatics tools have shown that VMPOMT has a conserved motif for O-methyltransferase group as found in OMT sequences from other plants that might be involved in methylation reactions in fragrance compounds biosynthesis in this orchid. Besides that, VMPOMT is predicted to be localised in cytoplasm instead of plastid due to the absence of chloroplast transit peptide in the sequence. Thus, sequence analyses on VMPOMT using several bioinformatics tools might contribute to the understanding of fragrance biosynthetic pathways in Vanda Mimi Palmer as well as in other fragrant orchids. Keywords: Vanda Mimi Palmer; fragrant-orchid; fragrant-related transcript; O-methyltransferase, RACE-PCR, vandaceous orchids.
Authors and Affiliations
Barudin Mohd Aiman, Ab. Rahim Mohd Hairul, Tan Suat Hian
Keywords
Related Articles
- EP ID EP378526
- DOI -
- Views 61
- Downloads 0
Morphometric Analysis of Supratrochlear Foramen of Humerus in South Indian Population
Olecranon and coronoid fossae of lower end of humerus separated by thin plate of bony septum (0.5 to 1 mm thickness). Sometimes the bony septum may shows perforated clear foramen is referred as Supratrochlear foramen (ST...
Normal Menstrual pattern profile
Menstrual cycle is a regular natural change in the female reproductive system which prepares the uterus for pregnancy. Commencement of menstrual cycle is considered as sign of puberty. The study was undertaken to know th...
Isolated Tubercular Osteomyelitis of Calcaneum: A Rare Case Report
Abstract: Tuberculosis is a leading cause of morbidity and mortality in developing countries including India. Skeletal tuberculosis accounts for 1-3% of all cases of tuberculosis. 10% of osteoarticular tuberculosis affec...
Adsorption of methylene blue dye from aqueous solution using activated carbon produced from water hyacinth in a fixed–bed column system.
This paper investigates the ability of activated carbon produced from water hyacinth to remove methylene blue dye from aqueous solution in a packed bed down-flow column. The experiments were performed with different bed...
Comparative potency of three insecticides against the infestation of brinjal shoot and fruit borer, Leucinodes orbonalis Guen.
Abstract: Brinjal shoot and fruit borer (BSFB), Leucinodes orbonalis Guenee, is a serious pest of brinjal or eggplant (Solanum melongena L.). Due to increasing levels of resistance of L. orbonalis to different insecticid...