Serological and molecular studies of ovine and human toxoplasmosis with a trial of treatment of infected ewes
Journal Title: Scientific Journal of Veterinary Advances - Year 2013, Vol 2, Issue 11
Abstract
The aims of the present study were to diagnose toxoplasmosisin pregnant ewes and women serologically and molecularly, treat naturallyinfected ewes, and diagnose congenital toxoplasmosis. Blood samples were taken from 30 and 60pregnant ewes and women, respectively, and used for diagnosis of toxoplasmosisthrough Latex agglutination test (LAT). Seropositive samples were confirmed byPCR for detection of acute infection. Ten infected pregnant ewes wereclassified into two groups. The first group was treated with sulfadimidine33.3%, 200 mg (0.6 ml) / kg.b.wt, and the other group was treated with normalsaline. At titers ≥ 1:64, serological diagnosis indicated that 16 (53.33%) ewesand 29 (48.3%) women were seropositive and the seroprevalence increased in olderewe and younger women. Positive LATsamples were used for amplification of DNA and showed bands at 193 bp,analogues to that of the RH strain in 12 (40%) and 15 (25%) blood samples ofewes and women, respectively. All treated ewes with sulfadimidine 33.3%delivered healthy lambs with normal gestation period, whereas untreated ewesdelivered 4 abortuses and 3 stillbirths. The tissue cysts were demonstratedmicroscopically in stained smears from tissues of dead fetuses. Local strain of T. gondii was isolatedthrough intra peritoneal injection in mice from tissues of abortuses andstillbirths and maintained in the lab. The DNA of both RH (a virulent strain) andthe local strains expressed diagnostic amplified DNA bands at 193 bp,indicating a zoonotic importance of T. gondii and the role of sheep a assource of human infection.
Authors and Affiliations
H. F. Khater*| Parasitology Department, Faculty of Veterinary Medicine, Benha University, Toukh, 13736, Egypt., N. O. Khalifa| Zoonoses Department, Faculty of Veterinary Medicine, Benha University, Toukh, 13736, Egypt., A. M. A. Barakat| Zoonoses Department, National Research Center, Dokki, 12622, Giza, Egypt.
Keywords
Related Articles
- EP ID EP899
- DOI 10.14196/sjvs.v2i11.1080
- Views 432
- Downloads 21
Bacteriological quality and occurrence of Escherichia coli and Staphylococcus aureus in ice cream sold in Zaria, Kaduna State Nigeria
A cross-sectional study was conducted across Zaria metropolis to determine the bacteriological quality and occurrence of Escherichia coli and Staphylococcus aureus in ice cream sold to consumers by supermarkets and stree...
Prevalence of gastrointestinal helminths on equines in and around Debre Markos, Ethiopia
The study was conducted from October-2013 to June-2014 in and around Debre Markos, Ethiopia to assess the major gastrointestinal helminthes of equines (donkeys and horses), to determine their prevalence and find associat...
Host range and transmission of Tobacco streak virus (TSV) causing cotton mosaic disease
Tobacco streak virus (TSV) causing cotton mosaic disease was found to be transmissible by mechanical means specially when extracts were made in neutral phosphate buffer 0.02M containing reducing agent like 2-Mercaptoetha...
Lactational cadmium exposure induced alterations in the hematological indices and oxidative status in brain, liver and testes of rat pups
This study was carried out to investigate the effect of lactational exposure to cadmium (Cd) on suckling male rat pups. Hematological parameters, antioxidant status, some biochemical and pathophysiological indices in br...
Some comparative gross and morphometrical studies on the gastrointestinal tract in pigeon (columbia livia) and Japanese quail (coturnix japonica)
To study the comparative morphology and morphometry of the gastrointestinal tract of the Japanese quail and pigeon, a total number of twenty birds twenty birds (comprising of ten pigeons and ten Japanese quails) of both...