Serological characteristics and molecular mechanisms of occult hepatitis B virus infection in blood donors
Journal Title: Chinese Journal of Blood Transfusion - Year 2024, Vol 37, Issue 11
Abstract
[Objective] Abstract: Objective To analyze the serological characteristics of occult hepatitis B virus infection (OBI) in blood donors, and to explore the molecular mechanism of HBV S gene mutation in the immune escape of hepatitis B virus surface antigen (HBsAg). [Methods] From January 2021 to December 2022, a total of 175 583 blood donors were recruited for ELISA/NAT testing. Subsequently, HBV serological marker testing was conducted to confirm the presence of OBI. The HBV S region was amplified using nested PCR and subjected to sequencing analysis. Comparative study was performed using MEGA 7.0 software. [Results] A total of 82 OBI samples were detected, with a detection rate of 0.046% (82/175 583). There was no statistically significant difference in the OBI detection rate among blood donors of different genders (P>0.05). The highest OBI detection rate among blood donors was observed in the age group of 45-54 years, accounting for 39.02%. HBcAb exhibited the highest positive rate at 52.44%. Sequencing analysis of HBV DNA in 12 OBI samples revealed that two cases had genotype B and 10 cases had genotype C. Nested PCR targeting the HBV S region identified amino acid mutations such as T47K, I126S and P127H in all 12 samples. [Conclusion] The implementation of NAT can effectively mitigate or even eliminate the risk of blood transfusion-associated OBI. The presence of high-frequency amino acid mutations, specifically T47K and Q101R, in the C genotype of OBI blood donors may lead to immune evasion, resulting in a negative HBsAg test.
Authors and Affiliations
Wei YU, Fang WANG, Qiang LIU
Keywords
Related Articles
- EP ID EP752552
- DOI 10.13303/j.cjbt.issn.1004-549x.2024.11.008
- Views 14
- Downloads 1
Secondary herpes zoster wound cured by allogeneic platelet-rich plasma: a case report
Objective To investigate the feasibility of allogeneic platelet-rich plasma (PRP) for the treatment of herpes zoster wounds secondary to systemic lupus erythematosus (SLE), especially large ulcer wounds. Methods The trea...
The applicability of Beckman PK7300 fully automatic blood group instrument for TPPA detection
Objective To investigate the applicability of Beckman PK7300 for TPPA testing on anti-TP reactive specimens from blood donors. Methods 1) The TPPA test using Beckman PK7300 (abbreviated as instrument method) had been est...
Potential value of differential expression of CDC42 gene in adult and umbilical cord blood reticulocytes
Objective To explore critical regulatory genes in the hemoglobin switch process by analyzing transcriptomic data from the GSE6236, GSE17639 and GSE35102 datasets. Methods The mRNA expression profiles of the three dataset...
Clinical transfusion consultation investigation and typical case analysis
Objective To analyze the characteristics of blood transfusion consultation cases and establish the consultation route, so as to provide reference for blood transfusion doctors to participate in blood transfusion consulta...
Optimization of quality assessment system of clinical blood transfusion records
Objective To determine the value of quality assessment system in supervising standard clinical blood use and improving the quality of clinical blood transfusion medical records. Methods The clinical blood transfusion rec...