Signal peptide of human nerve growth factor is better than signal peptide of mouse nerve growth factor in mediating secretory expression of beta-endorphin
Journal Title: Di-er Junyi Daxue Xuebao - Year 2009, Vol 30, Issue 4
Abstract
Objective:To clarify whether the signal peptide of human nerve growth factor can mediate secretory expression of beta-endorphin and whether there is difference between the efficiency of signal peptides from human and mouse nerve growth factor. Methods: Two kinds of eukaryotic vectors containing human or mouse signal sequence-mediated secretory expression of beta-endorphin were constructed.The culture supernatant and cells were collected 48 h after NIH3T3 cells were transfected by the two kinds of vectors, and the cover slips with single-layer cells was prepared. The concentration of beta-endorphin in the culture was determined by radio-immunoassay. The total RNA was extracted from cells and mRNA from fusion genes was assayed by RT-PCR. Cells on cover slips were subjected to immunofluorescence staining. Results: RT-PCR showed that the fusion genes were expressed in NIH3T3 cells; the expression of beta-endorphin was mainly in the cytoplasm of NIH3T3 cells. The concentrations of beta-endorphin in the supernatants 48 h after transfection with pcDNA3.1-hEP and pcDNA3.1-mEP were (280.33±24.16) pg/ml and (191.04±7.96) pg/ml (P<0.05), respectively, and they were significantly different from that of the blank control group (P<0.01). Conclusion: The signal sequence of human nerve growth factor can mediate the secretory expression of protein and the efficacy of human signal peptide is higher than that of mouse signal peptide.
Authors and Affiliations
Xue-wu XU, Shu-jun PEI, Xue-rong MIAO, Wei-feng YU
Keywords
Related Articles
- EP ID EP84371
- DOI -
- Views 118
- Downloads 0
The development of quorum sensing phenomenon in pathogenic fungi
The quorum sensing commonly exists in procaryote kingdom, regulating various biologic functions. Recently similar phenomenon was also found in fungi world, which affecting both biofilm formation and dimorphism. In this a...
Change of immunological response and cell proliferation in breast augmented by polyacrylamide hydrogel injection
Objective：To study the change of immunological response and cell proliferation in breast tissues augmented by polyacrylamide hydrogel injection(PHI).Methods:The expression of CD68,CD25 and PCNA in 20 breast tissues with...
Application of ultrasmall superparamagnetic iron oxide in MRI diagnosis of central nervous system:recent progress
Ultrasmall superparamagnetic iron oxide (USPIO),as the contrast agent of MRI,possesses two major properties:long half time in the plasma and specific binding with macrophages.Compared with gadolinium,widely-used in clini...
Clinical analysis of disease-associated nasal anatomic abnormality in 328 cases of chronic dacryocystitis
透皮促渗剂对双乌跌打损伤药方中胡椒碱体外经皮渗透的影响
目的:研究不同透皮促渗剂对复方跌打损伤药方中主要成分胡椒碱体外经皮渗透的促进作用。方法:采用Franz扩散池,选择氮酮、丙二醇、N-甲基吡咯烷酮（NMP）、油酸、薄荷油及其组合作为透皮促渗剂,以预处理的离体小鼠皮肤作为渗透屏障,HPLC 法...