Silver tolerance and silver nanoparticle biosynthesis by Neoscytalidium novaehollandae and Trichoderma inhamatum
Journal Title: European Journal of Biological Research - Year 2016, Vol 6, Issue 1
Abstract
Development of reliable and eco-friendly processes for synthesis of metallic nanoparticles is an important step in the field the applications of nanotechnology. Biological systems provide a useful option to achieve this objective. In this study, Twenty-one silver tolerating fungal species and 3 varieties belonging to 8 genera were collected from 40 soil samples, the most common genus was Aspergillus was recovered from 97.5% of the samples on silver supplemented medium, followed by silver tolerance (1 mM and 2 mM) test. The highest two silver tolerant isolates were subjected to rRNA gene sequencing and were confirmed as Neoscytalidium novaehollandae and Trichoderma inhamatum. The fungal cell filtrates of these two fungi were incubated with AgNO3. The visual observation of brownish color is an indication of silver nanoparticle production. Further characterization was made by UV-visible absorption spectroscopy which showed maximum absorption at 430 and 453 nm for N. novaehollandae and T. inhamatum, respectively. Transmission Electron Microscope (TEM) revealed the formation of spherical nanoparticles with particles size from 4-20 and 2-15 nm for both fungi, respectively. The synthesized silver nanoparticles were exhibited antibacterial activities against pathogenic some bacterial strains which causes the diseases in human.
Authors and Affiliations
Mohamed Hussein
Keywords
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