Simultaneous Determination of Cefepime, Cefotaxime and Ceftriaxone in Pharmaceutical formulation by Ultra-Fast Liquid Chromatography with PDA Detection
Journal Title: IOSR Journal of Pharmacy and Biological Sciences (IOSR-JPBS) - Year 2018, Vol 13, Issue 5
Abstract
A simple, rapid and accurate Ultra-fast liquid chromatography (UFLC) method was developed for simultaneous determination for cefepime, cefotaxime and ceftriaxone in pharmaceutical formulation. The chromatographic separation was conducted on UFLC Shimadzu connected with PDA detector; using column Phenomenex, Prodigy, ODS3, 5 µm, (250 x 4.6 mm) with a mobile phase was isocratic consisted of Acetonitrile and 0.025M KH2PO4 pH 3.0 in the ratio of 15:85 (by volume) and was delivered to the system at a flow rate of 1.5 ml/min. An injection volume of 20 µl was used for cefepime, cefotaxime and Ceftriaxone. The detection wavelength (λ max) was 260 nm for cefepime, cefotaxime and Ceftriaxone. The calibration curve of cefepime in mobile phase was linear with correlation coefficient (r2) = 0.9998; over a concentration range of 25.0 – 100.0 mg/L for; with a retention time of 2.37 minutes, the calibration curve of cefotaxime in mobile phase was linear with correlation coefficient (r2) = 0.9993; over a concentration range of 50.0 – 200.0 mg/L for; with a retention time of 5.90 minutes. While the calibration curve of Ceftriaxone in mobile phase was linear with correlation coefficient (r2) = 0.9974; over a concentration range of 100.0 – 400.0 mg/L for; with a retention time of 4.15 minutes. The relative standard deviation (RSD) was found to be < 2. The validated HPLC method was successfully applied to the analysis for cefepime, cefotaxime and Ceftriaxone in pharmaceutical dosage form.
Authors and Affiliations
H. E. El- Beltagy, A. S. Amin, M. N. El- Balkeny, S. A. Madkour
Keywords
Related Articles
- EP ID EP415646
- DOI 10.9790/3008-1305035461.
- Views 77
- Downloads 0
Etiological Study of Peripheral Oculomotor Palsy : About 50 Cases / Etude Étiologique Des Paralysies Oculomotrices Périphériques: A Propos De 50 Observations
.
In Vitro and In Vivo Hepatoprotective Activity of Madhuca Longifolia L Leaves on Carbon Tetrachloride Induced Rats
Administration of Madhuca longifolia Methanolic Extract (MLME) and derivative of Madhucic Acid (dMA) a bioactive from it leaves and standard drug silymarin in rats showed significant hepatoprotective action against CCl4...
Studies on Micromorphological Taxonomic Variations in Abutilon Species of Indian Thar Desert
Micromorphological taxonomic variations of leaves in three common species of genus Abutilon Mill.viz. Abutilon indicum(Linn.) Sweet,Abutilon pannosum (Forst.f.) Schlect. and Abutilon ramosum (Cav.) Guill. and Perr. in In...
Inhibition of Fungal Growth And Mycotoxin Productions Using Lactic Acid Bacteria Isolated From Milk And Fermented Food
Twenty one bacterial isolates were obtained from the normal habitats of lactic acid bacteria (LAB). They were isolated from women milk, sheep milk, yoghurt, pasterilized milk and fermented food" pickles". Screening of al...
Antimicrobial Activity of Cinnamonextracts against Foodborne Pathogens E.Coli, S.Tyhimurium and S.Aureus & L.Monocytogens
Solvents extracts of Cinnamon (Cinnamomum cassia) were analyzed for determination of antibacterial activity against four food borne pathogens: (4strains), two gram negative, Escherichia coli, Salmonella typhi, and two gr...