Simultaneous Determination of Cefepime, Cefotaxime and Ceftriaxone in Pharmaceutical formulation by Ultra-Fast Liquid Chromatography with PDA Detection
Journal Title: IOSR Journal of Pharmacy and Biological Sciences (IOSR-JPBS) - Year 2018, Vol 13, Issue 5
Abstract
A simple, rapid and accurate Ultra-fast liquid chromatography (UFLC) method was developed for simultaneous determination for cefepime, cefotaxime and ceftriaxone in pharmaceutical formulation. The chromatographic separation was conducted on UFLC Shimadzu connected with PDA detector; using column Phenomenex, Prodigy, ODS3, 5 µm, (250 x 4.6 mm) with a mobile phase was isocratic consisted of Acetonitrile and 0.025M KH2PO4 pH 3.0 in the ratio of 15:85 (by volume) and was delivered to the system at a flow rate of 1.5 ml/min. An injection volume of 20 µl was used for cefepime, cefotaxime and Ceftriaxone. The detection wavelength (λ max) was 260 nm for cefepime, cefotaxime and Ceftriaxone. The calibration curve of cefepime in mobile phase was linear with correlation coefficient (r2) = 0.9998; over a concentration range of 25.0 – 100.0 mg/L for; with a retention time of 2.37 minutes, the calibration curve of cefotaxime in mobile phase was linear with correlation coefficient (r2) = 0.9993; over a concentration range of 50.0 – 200.0 mg/L for; with a retention time of 5.90 minutes. While the calibration curve of Ceftriaxone in mobile phase was linear with correlation coefficient (r2) = 0.9974; over a concentration range of 100.0 – 400.0 mg/L for; with a retention time of 4.15 minutes. The relative standard deviation (RSD) was found to be < 2. The validated HPLC method was successfully applied to the analysis for cefepime, cefotaxime and Ceftriaxone in pharmaceutical dosage form.
Authors and Affiliations
H. E. El- Beltagy, A. S. Amin, M. N. El- Balkeny, S. A. Madkour
Keywords
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- EP ID EP415646
- DOI 10.9790/3008-1305035461.
- Views 63
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