Simultaneous Determination of Salbutamol Sulphate and Ambroxol Hydrochloride in Solid Dosage Form by RP-HPLC
Journal Title: International Journal of Pharma Research and Health Sciences - Year 2014, Vol 2, Issue 6
Abstract
The A new reverse phase high performance liquid chromatographic method for the simultaneous determination of salbutamol and ambroxol in tablet form has been developed and validated. Salbutamol and ambroxol are frequently associated with bronchospasm. The separation was carried out by using a mobile phase which consists of methanol and 0.1 percent triethylamine at pH 3.0 (50:50). The study was carried out on Younglin isocratic HPLC System having UV detector at the wavelength of 224 nm. The stationary phase used was Cosmosil c-18 column having 4.6 mm inner diameter, 250 mm length and particle size of 5 μm, the column temperature was maintained at 25° C and injection volume of 20 μl. The retention time were found to be 3.5 minutes for ambroxol and 6 minutes for salbutamol. The peaks were well resolved and the percent recovery of salbutamol and ambroxol were within limit of 98.0 % to 102.0 %. The developed method was accurate, reproducible and therefore suitable for routine analysis.
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