Abstract

A simple and rapid reversed phase-high performance liquid chromatographic method was developed for simultaneous determination of Irbesartan and Hydrochlorthiazide in Tablet Dosage form. Method: The elution was done with a mobile phase of Methanol:0.05 % Orthophosphoric acid (90:10) on Intersil-BDS C18 column (250 × 4.6 mm, 5 μm particle size). The wavelength detector was set at 226 nm. Results and discussion: Retention times for Irbesartan and Hydrochlorthiazide were around 2.869 min, 3.942 min respectively. The reliability and analytical performance of the proposed HPLC procedure were statistically validated according to the respect of linearity, ranges, precision, accuracy, repeatability, reproducibility, detection and quantification limits. Linear ranges were established between 36-216 μg/mL for Irbesartan and 3-18 μg/mL for Hydrochlorthiazide. The LOD and LOQ for Irbesartan were found to be 0.65, 1.97 and for Hydrochlorthiazide were found to be 0.513, 1.556 respectively. The described High Performance Liquid Chromatography method was successfully employed for the analysis of pharmaceutical formulations containing combined dosage form.

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