SIMULTANEOUS ESTIMATION OF GUGGULSTERONE E, GUGGULSTERONE Z, KBA, AKBA, WITHAFERIN A AND 6-GINGEROL BY USING HPLC-DAD METHOD FROM ARIFLEX TABLET FORMULATION
Journal Title: International Journal of Pharmaceutical Sciences and Drug Research - Year 2015, Vol 7, Issue 1
Abstract
Simple, accurate, precise, sensitive and validated HPLC method was developed for simultaneous determination of Guggulsterone E (GE), Guggulsterone Z (GZ), 11-keto-β-boswellic acid (KBA), 3- Acetyl 11-keto-β-boswellic acid (AKBA), Withaferin A (WA) and 6-Gingerol (GNRL) in Ariflex tablet. The gradient RP-HPLC analysis was performed on Inertsil ODS-3V C18 column (250 × 4.6 mm id), using a mobile phase consisting of 0.1% Ortho-phosphoric acid and mixture of acetonitrile: Methanol (50: 50 v/v) in solvent gradient elution for 60 min at a flow rate of 1.0 mL min−1. Quantification was carried out using a photodiode array detector at 225nm. The employment of diode array detector allowed selectivity confirmation by peak purity evaluation. The method was validated according to International Conference on Harmonization guidelines. No chromatographic interference from tablet excipients was found and hence this method is applicable for simultaneous determination of GE, GZ, KBA, AKBA, WA and GNRL in Ariflex Tablet.
Authors and Affiliations
Pankaj Patil, Sanjay Nipanikar, Hanumant Bhusnar, Dheeraj Nagore
Keywords
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