SIMULTANEOUS ESTIMATION OF TELMISARTAN AND HYDROCHLOROTHIAZIDE BY DERIVATIVE SPECTROSCOPY
Journal Title: International Journal of Pharmacy and Pharmaceutical Sciences - Year 2015, Vol 7, Issue 6
Abstract
Objective: The objective of this investigation is to present the simple and sensitive method for the analysis of Telmisartan and Hydrochlorothiazide and apply the proposed method for the analysis of said drugs in pharmaceutical formulations.Methods: Telmisartan and Hydrochlorothiazide are used in combination for treatment of hypertension. The present work deals with simple derivative spectrophotometric method development for simultaneous estimation of Telmisartan and Hydrochlorothiazide in two component tablet formulation.Results: The method employed first order derivative spectroscopy for determination of ʎmax by taking 10 μg/ml each of Telmisartan and Hydrochlorothiazide were scanned in 200-400 nm range and ʎmax were observed 291 nm for Telmisartan and Hydrochlorothiazide showed zero crossing point and 251 nm for Hydrochlorothiazide and Telmisartan showed zero crossing point in first order derivative spectroscopy.Conclusion: For this method, linearity was observed in 2-40 μg/ml for Telmisartan and 2-20 μg/ml for Hydrochlorothiazide. The recovery study confirmed the accuracy of the proposed method and low values of standard deviation confirmed precision of the used method. The method was validated as per ICH guidelines.
Authors and Affiliations
Piusha Shakya, Pushpendra Kumar Jain, Asmita Gajbhiye, S. P. Shrivastava
Keywords
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