Simultaneous Extraction and Detection of Six Fungicide Residues in Mango Fruit Followed by New Validated HPLC-UV Method
Journal Title: Scholars Academic Journal of Biosciences - Year 2013, Vol 1, Issue 3
Abstract
A simple, sensitive and inexpensive method was developed using matrix solid-phase dispersion (MSPD), together with high performance liquid chromatographic method with uv detection for determination of triazole fungicide residues (Tricyclazole, Hexaconazole, Difenoconazole) and strobilurin fungicide residues (Azoxystrobin, Trifloxystrobin, Picoxystrobin) in Mango fruit. The evaluated parameters include the type and amount of sorbent (silica gel, C18 and Activated Florisil) and the nature of eluent (ethyl acetate, dichloromethane and acetonitrile).The best results were obtained using 2.0 g of Mango fruit sample, 2.0 g of C18 as sorbent and 20ml of ethyl acetate-dichloromethane (1:1, (v/v)).The method was validated using in Mango fruit samples spiked with fungicides at different concentration levels (0.03 and 0.3 µg/mL). Average recoveries (using each concentration six replicates) ranged 90-97%, with relative standard deviations less than 2%, calibration solutions concentration in the range 0.01-2.0 µg/mL and limit of detection (LOD) and limit of quantification (LOQ) were 0.01 µg/mL and 0.03 µg/mL respectively. Keywords: matrix solid-phase dispersion, triazole fungicides, strobilurin fungicides, HPLC-UV, LOQ, LOD.
Authors and Affiliations
Tentu. Nageswara Rao, D. Sreenivasulu,, B. Patrudu, E. G. Sreenivasula Reddy
Keywords
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