Single nucleotide polymorphisms of the human M1 muscarinic acetylcholine receptor gene
Journal Title: The AAPS Journal - Year 2001, Vol 3, Issue 4
Abstract
The gene encoding the human muscarinic receptor, type 1 (CHRM1), was genotyped from 245 samples of the Coriell Collection (Coriell Institute for Medical Research, Camden, NJ). Fifteen single nucleotide polymorphisms (SNPs) were discovered, 9 of which are located in the coding region of the receptor. Of these, 8 represent synonymous SNPs, indicating that CHRM1 is highly conserved in humans. Only a single allele was found to contain a nonsynonymous SNP, which encodes an amino acid change of Cys to Arg at position 417. This may have functional consequences because a C417S point mutation in rat M1 was previously shown to affect receptor binding and coupling. Furthermore, 0 of 4 SNPs within CHRM1 previously deduced from sequencing of the human genome were found in this study despite a prediction that a majority of such inferred SNPs are accurate. The consensus sequence of CHRM1 obtained in our study differs from the deposited reference sequence (AC {"type":"entrez-nucleotide","attrs":{"text":"NM_000738","term_id":"1519244118","term_text":"NM_000738"}} NM_000738) in 2 adjacent nucleotides, leading to a V173M change, suggesting a sequencing error in the reference sequence. The extraordinary sequence conservation of the CHRM1 gene-coding region was unexpected as M1-knockout mice show only minimal functional impairments.
Authors and Affiliations
Julie L. Lucas, Wolfgang Sadee, Joseph A. DeYoung
Keywords
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- EP ID EP682068
- DOI 10.1208/ps030431
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