Single strand conformation polymorphisms (SSCPs) in the 5'- flanking region of Lactoferrin gene and its association with milk production traits and somatic cell score in Holstein cattle
Journal Title: International journal of Advanced Biological and Biomedical Research - Year 2014, Vol 2, Issue 2
Abstract
Lactoferrin (Lf) is a bioactive protein present in all external secretions such as milk that plays important role in the innate host defense. Therefore, the Lf has a major role in bovine mammary gland defense during mastitis. In this study, 5’-flanking region of bovine Lf gene containing three single nucleotide polymorphisms (SNPs) at positions −602 (T/G), −600 (A/G) and -586 (T/C) were screened using PCRSSCP method. Three distinct SSCP patterns, representing 3 different haplotypes (A, B and C), were identified. The frequencies of A, B, and C haplotypes for amplified fragment were 0.43, 0.39 and 0.18, respectively. An association study was applied with data from milk production traits and somatic cell score (SCS). Statistically significant differences were found between various haplotypes at the amplified fragment and fat percentage (P<0.05). However, the haplotypes also tend to associate with protein percentage (P=0.06) and milk yield (P=0.08). No significant difference was observed between combined genotypes and SCS. Therefore, the haplotypes identified in the 5'- flanking region of bovine Lf gene can serve as potential candidate genetic markers for marker assisted selection in cattle.
Authors and Affiliations
Mustafa Muhaghegh-Dolatabady| Department of Animal Science, Faculty of Agriculture, University of Yasouj, Iran, E-mail: mmuhaghegh@yu.ac.ir, Aria Shafaeipour| Department of Animal Science, Faculty of Agriculture, University of Yasouj, Iran
Effects of inter and intra row spacing on growth, yield and yield components of Roselle (Hibiscus sabdariffa L.)
A field experiment was conducted to assess the optimum inter- and intra-row spacing on growth, yield and yield component of Roselle (Hibiscus sabdariffa L.) in 2014/15 cropping season at Wondo Genet Agricultural Resear...
Isolation and characterization of phenol degrading bacteria from Persian Gulf
Phenol and phenol compounds are environmental pollutants present in industrial wastewaters such as, coal tar, oil refineries and petrochemical plants exist. Phenol removal from industrial effluent is extremely importan...
Effects of the Aromatase Inhibitor, Letrozol, on Levels of Serum 17β-Estradiol (E2) And Enzymes in Rainbow Trout (Oncorhynchus Mykiss Walbaum) Females
In the present study, changes of values of serum 17ß-estradiol (E2), Asparate amino teransferase (AST), Alanine amino transferase (ALT) and Lactate dehydrogenase (LDH) following intraperitoneal (i.p) injection with th...
REPRODUCTIVE PERFORMANCE OF RAMS TO BIOSTIMULATION
The bio-stimulation technique offers a potentially useful and practical way for reproductive performance in rams. Reports on how bio-stimulation affects sexual potency rams are scarce: Knowledge of how rams respond to...
CONTRACEPTIVE EFFICACY OF HYDRO-METHANOLIC FRUIT EXTRACT OF XYLOPIA AETHIOPICA IN MALE ALBINO RATS
The rising human population, particularly in the developing countries, and the attendant socioeconomic effects necessitates the need for its effective regulation. This study aims at exploring the contraceptive efficacy...