SOLID PHASE MICROBIAL FERMENTATION OF ANABOLIC STEROID, DIHYDROTESTOSTERONE WITH ASCOMYCETE FUNGUS FUSARIUM OXYSPORUM
Journal Title: International Journal of Pharmacy and Pharmaceutical Sciences - Year 2015, Vol 7, Issue 4
Abstract
Objective: Microbial catalysis is used in the commercial production of many bioactive steroids. Solid phase microbial fermentation of anabolic steroid, dihydrotestosterone (DHT, 1), was carried out with ascomycete fungal strain Fusarium oxysporum (NRRL-1392).Methods: Sabouraud-4% glucose-agar was used to cultivate the fungal cultures as solid phase medium. Substrate 1 was incubated with Fusarium oxysporum (NRRL-1392) for 8 days. Microbial transformed metabolites were purified by using column chromatographic technique. Results: Ascomycete fungal strain Fusarium oxysporum (NRRL-1392), transformed dihydrotestosterone (1) to four oxidative metabolites 2-5 Â using solid phase microbial transformation metod. During biotransformation process the hydroxy group was incorporated in inactivated methine carbon atoms at C-7 and C-11 positions. Their structures were elucidated by means of a homo and heteronuclear 2D NMR and by HREI-MS techniques as 17b-hydroxyandrosta-1, 4-dien-3-one 2, androsta-1, 4-diene-3, 17-dione 3, 7a, 17b-dihydroxyandrosta-1, 4-dien-3-one (4), and 11a-hydroxyandrosta-1, 4-diene-3, 17-dione 5. The relative stereochemistry of newly incorporated hydroxy groups were deduced by 2D NOESY experiment.Conclusion: In conclusion, microbial biocatalysis is an attractive alternative tool for the preparation of new bioactive steroids, which might be difficult to prepare by conventional chemical routes. Furthermore, microbial-catalyzed biotransformations can produce commercially valuable steroidal pharmaceuticals for the pharmaceutical industry.Â
Authors and Affiliations
Muhammad Atif, Syed Adnan Ali Shah, Sadia Sultan, Muhammad Iqbal Choudhary
CYTOPROTECTIVE EFFECT OF OCIMUM EXTRACT ON INJURED RENAL EPITHELIAL CELLS
Objectives: Renal cell injury is one of the important factors affecting urolithiasis. Crystals which aggregate to constitute the kidney stones are formed in the supersaturation condition in kidney. Reactive oxygen specie...
ANTIMICROBIAL EFFECTS OF INDONESIAN MEDICINAL PLANTS EXTRACTS ON PLANKTONIC AND BIOFILM GROWTH OF PSEUDOMONAS AERUGINOSA AND STAPHYLOCOCCUS AUREUS
Objective: The increasing rates of antibiotic-resistant microbial infections requires continuous development of new antimicrobial agents. Moreover, microbial biofilms exhibit elevated resistance to most antimicrobial dru...
SMEDDS TABLET: COMPATABILITY OF SOLID SMEDDS USING VARIOUS PHARMACEUTICAL TABLET EXCIPIENTS
Objective: There are many successful products on the market which are the culmination of the self-micro-emulsification lipid technology applications. Despite the importance of lipid-based formulations, these systems have...
SOLUBILITY ENHANCEMENT OF NORFLOXACIN BY HYDROTROPY TECHNIQUE
The objective of this study is to improve the solubility of poorly water soluble anti-bacterial drug Norfloxacin by hydrotropic solubilization technique. For the present study sodium benzoate was used as hydrotropic agen...
A GLOBAL COMPARISON AND ANALYSIS OF NEURAMINIDASE H1N1 STRAIN OF INFLUENZA A
Objective: To evaluate the variation of neuraminidase (NA) protein from various H1N1 strain for drug designing.Methods: In this study we have used 12 sequences of NA protein from various countries, retrieved from Uniprot...