Somatic embryo differentiation and germination of Coffea arabica L. cv. Catimor 9722 obtained in an orbital shaker
Journal Title: Biotecnología Vegetal - Year 2005, Vol 5, Issue 2
Abstract
The present work had as objectives to study the effect of four inoculation densities on the process of differentiation of embryogenic cell suspensions of Coffea arabica cv. Catimor 9722 and to evaluate the effect of two culture media described in the literature for the germination of somatic embryos. The results showed that somatic embryos were only formed in the treatments inoculated with 0.5 and 1.0 g FW l -1 , while in the treatments inoculated with 5.0 and 10.0 g FW l -1 only the cell aggregates were multiplied. The largest number of somatic embryos (23 154 SE.l -1 ) was obtained when inoculating 1.0 g FW l -1 , with statistical differences regarding the treatment inoculated with 0.5 g FW l -1 , in which 12 507 SE.l -1 were obtained. Regarding the germination of the somatic embryos in torpedo phase, the best results were possible when using 0.225 mg.l -1 de 6-BAP, because after eight weeks of culture they germinated with an average of 2.87 somatic embryos out of five placed initially in the culture flasks, what represented 57.5% of germination.
Authors and Affiliations
Manuel de Feria Silva, Elio Jiménez González, Raúl Barbón Rodríguez, Alina Capote Pérez, Maité Chávez Milián, Elisa Quiala Mendoza
Keywords
Related Articles
- EP ID EP684238
- DOI -
- Views 126
- Downloads 0
Embryogenic callus formation in Dioscorea rotundata Poir cv. ‘Blanco de Guinea’
Yam contributes to energy and nutritional requirements of most of the population of developing countries. However, their extensive culture is constrained by the limited availability of planting material with physiologic...
Effect of inoculum density on the morphology and physiology of Bambusa vulgaris Schrad. ex Wendl shoots cultured in Temporary Immersion System
Bambusa vulgaris Schrad. Ex Wendl is a species of great environmental importance and plays a vital role in the economic development of Cuba. The present work was carried out with objective of determining effect of inoc...
Genomic characterization by AFLP of Stevia rebaudiana Bertoni cultivated in the department of Antioquia (Colombia)
Stevia rebaudiana Bertoni, Asteraceae family (2n = 22), is a plant native from Paraguay, it has in leaves tissues a high production of some substances with strong edulcorant power, getting to be 320 sweet times but that...
Influence of magnetic field on the in vitro establishment of zygotic embryos of Adenanthera pavinina L.
The in vitro embryo culture for pre germinal treatment allows the application of low frequency electromagnetic and magnetic fields. The species Adenanthera pavonina L. has seeds with a waterproof integument, long laten...
Effect of synthetic brassinosteroids on the induction of rice callus
The present work shows the effect of the synthetic brassinosteroids BIOBRAS 6, ME and BIOBRAS 16 in the induction of callus of rice (Oryza sativa L) of the commercial varieties J-104 and Pokkali. Mature seeds were used...