Speciation and antibiotic resistance pattern of Acinetobacter species in a tertiary care hospital in Uttarakhand
Journal Title: International Journal of Medical Research & Health Sciences (IJMRHS) - Year 2016, Vol 5, Issue 1
Abstract
Acinetobacter species are Gram negative nonfermentative bacteria that have now emerged as important nosocomial pathogens involved in outbreaks of hospital infections.They are considered as opportunistic pathogens that readily colonize patients with compromised host defenses especially in intensive care units (ICUs), neonatal units, and surgical wards. The current study was conducted to type the Acinetobacter isolates obtained from various sources by a simplified phenotypic identification scheme and also to determine their antimicrobial susceptibility. Specimens like blood, CSF, endotracheal aspirate, urine, sputum, pus, bronchoalveolar lavage, HVS and body fluids were processed by standard methods and the antibiotic-sensitivity was performed by Kirby-Bauer disk diffusion technique as per Clinical and Laboratory Standards Institute guidelines (CLSI). The study was conducted in a tertiary care hospital for a period of 6 months (July 2013 – Dec 2013) in which out of a total of 1272 culture positive specimens, 53 Acinetobacter isolates were obtained from various specimens. Speciation was done in which predominance of A. baumannii( 90.6%) was seen while A. lwoffii and A. haemolyticus showed an isolation rate of 5.7% and 3.8% respectively. High levels of resistance were seen for Ampicillin –Sulbactam (96%), Ampicillin (94%), Aztreonam (94%), Cefuroxime (92%), ceftazidime (91%). The p-value was found to be statistically significant for all the above mentioned antibiotics except for Polymyxin B for which 100% sensitivity was recorded. Clinical co-relation must be under taken to exclude commensal contaminants, before considering it to be a pathogen and prescribing antibiotics to the patient.
Authors and Affiliations
Raina Dimple| Department of Microbiology Shri Guru Ram Rai Institute of Medical and Health Sciences, Dehradun, India, Corresponding email: dr.dimpleraina@gmail.com, Sharma Nupur| Department of Microbiology, AIIMS , Delhi, India, Mahawal B. S. | Department of Microbiology Shri Guru Ram Rai Institute of Medical and Health Sciences, Dehradun, India, Khanduri Ankit| Department of Microbiology Shri Guru Ram Rai Institute of Medical and Health Sciences, Dehradun, India, Pandita Ajay | Department of Community Medicine Shri Guru Ram Rai Institute of Medical and Health Sciences, Dehradun, India
Keywords
Related Articles
- EP ID EP11540
- DOI -
- Views 279
- Downloads 8
USE OF PROTON PUMP INHIBITORS IN GENERAL PRACTICE: IS IT RATIONALE?
Background: The incidence of improper use of PPIs varies from 40-70% in various studies. Initiation and the continuous use of these drugs without correct indications will result in significant cost to the patient. The p...
Effect of the use of a safety intravenous catheters to prevent needle stick injuries
The damage caused by sharp objects is one of the most important biological hazards among health care workers. Due to the importance of occupational injury, this study aimed to investigate the efficiency of using safety...
Te Relation Between Laparoscopic Sleeve Gastrectomy and Ghrelin
Recently, obesity has become an important worldwide health problem. One of the obesity treatment alternatives is bariatric surgery methods and their effciency is increasing from day to day. In laparoscopic Sleeve gastre...
Short Term Effects of Neurodynamic Stretching and Static Stretching Techniques on Hamstring Muscle Flexibility in Healthy Male Subjects
Flexibility is a key component of rehabilitation and inadequate muscle extensibility remains a commonly accepted factor for musculoskeletal disorders. Studies on the most optimal technique for improving muscle flexibili...
Surgically treated early stage cervical cancer – Prognostic factors and adjuvant treatment
Invasive cancer of cervix is considered a preventable disease as it has a long pre-invasive state. If detected in early stages i.e upto stage IIA1 the disease is curable with good survival rates. • To identify the pr...