Spectrophotometric determination of Ceftiofur in pharmaceutical formulations by FGFCF, SFNO and MB
Journal Title: Journal of Pharmaceutical Sciences and Research - Year 2010, Vol 2, Issue 9
Abstract
Three simple spectrophotometric methods are proposed for the determination of Ceftiofur (CEFT) in bulk drug and in pharmaceutical preparations. The method is based on oxidation product with Fast green FCF (FGFCF)/KMnO4-, and ion association complex with safranin O (SFNO) and Methylene blue (MB). These two methods yield good results, included evaluation of the range, linearity, precision, accuracy, recovery, and specificity. The spectrophotometric determinations were performed at 625 (FGFCF), 530 (SFNO) and 655 nm (MB). A prospective validation showed that the methods are linear (r = 0.9999) and precise. The methods were successfully applied to the assay of CEFT in tablet preparations and the results were statistically compared with those of the reference method by applying the Student’s t-test and F-test. No interference was observed from the common tablet excipients. The accuracy of the method was further ascertained by performing recovery studies via standard addition method.
Authors and Affiliations
Medikondu Kishore , K. Surendrababu , Ch. S. R. G. Kalyani , M. Janardhan
Keywords
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