SPECTROPHOTOMETRIC ESTIMATION OF GATIFLOXACIN SESQUIHYDRATE AND PREDNISOLONE ACETATE IN COMBINED PHARMACEUTICAL DOSAGE FORM BY Q-ABSORBANCE RATIO METHOD
Journal Title: International Research Journal of Pharmacy (IRJP) - Year 2013, Vol 4, Issue 1
Abstract
The present manuscript describes simple, sensitive, rapid, accurate, precise and economical Q-absorbance ratio method for the simultaneous determination of Gatifloxacin sesquihydrate and Prednisolone acetate in mixture. Absorbance ratio method uses the ratio of absorbance at two selected wavelengths, one which is an isoabsorptive point and other being the λ-max of one of the two components. Prednisolone acetate and Gatifloxacin sesquihydrate show an isoabsorptive point at 267.20 nm in methanol. The second wavelength used is 243 nm, which is the λ-max of Prednisolone acetate in methanol. The linearity was obtained in the concentration range of 2-24 μg/ml for both Prednisolone acetate and Gatifloxacin sesquihydrate. The concentrations of the drugs were determined by using ratio of absorbances at isoabsorptive point and at the λ-max of Prednisolone acetate. The method was successfully applied to pharmaceutical dosage form because no interference from the synthetic mixture excipients was found. The suitability of this method for the quantitative determination of Prednisolone acetate and Gatifloxacin sesquihydrate was proved by validation. The proposed method was found to be simple and sensitive for the routine quality control application of Prednisolone acetate and Gatifloxacin sesquihydrate in mixture or pharmaceutical dosage form. The results of analysis have been validated statistically and by recovery studies.
Authors and Affiliations
Sejal Patel , Hina Patel
Keywords
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