SPECTROPHOTOMETRIC ESTIMATION OF GATIFLOXACIN SESQUIHYDRATE AND PREDNISOLONE ACETATE IN COMBINED PHARMACEUTICAL DOSAGE FORM BY Q-ABSORBANCE RATIO METHOD
Journal Title: International Research Journal of Pharmacy (IRJP) - Year 2013, Vol 4, Issue 1
Abstract
The present manuscript describes simple, sensitive, rapid, accurate, precise and economical Q-absorbance ratio method for the simultaneous determination of Gatifloxacin sesquihydrate and Prednisolone acetate in mixture. Absorbance ratio method uses the ratio of absorbance at two selected wavelengths, one which is an isoabsorptive point and other being the λ-max of one of the two components. Prednisolone acetate and Gatifloxacin sesquihydrate show an isoabsorptive point at 267.20 nm in methanol. The second wavelength used is 243 nm, which is the λ-max of Prednisolone acetate in methanol. The linearity was obtained in the concentration range of 2-24 μg/ml for both Prednisolone acetate and Gatifloxacin sesquihydrate. The concentrations of the drugs were determined by using ratio of absorbances at isoabsorptive point and at the λ-max of Prednisolone acetate. The method was successfully applied to pharmaceutical dosage form because no interference from the synthetic mixture excipients was found. The suitability of this method for the quantitative determination of Prednisolone acetate and Gatifloxacin sesquihydrate was proved by validation. The proposed method was found to be simple and sensitive for the routine quality control application of Prednisolone acetate and Gatifloxacin sesquihydrate in mixture or pharmaceutical dosage form. The results of analysis have been validated statistically and by recovery studies.
Authors and Affiliations
Sejal Patel , Hina Patel
Keywords
Related Articles
- EP ID EP146079
- DOI -
- Views 79
- Downloads 0
SIMULTANEOUS ESTIMATION AND VALIDATION OF PARACETAMOL, CHLORPHENIRAMINE MALEATE, PHENYLEPHRINE HYDROCHLORIDE, CAFFEINE AND ITS STRESS DEGRADATION STUDIES USING UV VISIBLE SPECTROSCOPY
A simple, precise, accurate and economic simultaneous UV spectrophotometric method has been developed for the estimation of Paracetamol, Chlorpheniramine Maleate, Phenylephrine Hydrochloride and Caffeine in tablet formul...
DETERMINATION OF ANDROGRAPHOLIDE IN ANDROGRAPHIS PANICULATA EXTRACTS WITH AND WITHOUT HUMAN SERUM BY HIGH PERFORMANCE THIN LAYER CHROMATOGRAPHY
Andrographis paniculata (Acanthaceae)commonly known as Kalmegh is used both in Ayurvedic and Unani system of medicines for a number of ailments. Kalmegh contains bitter principles – Andrographolide, 14-deoxy-11, 12- dide...
ELEMENTAL ANALYSIS OF Aerva lanata (L.) BY EDX METHOD
Trace elements concentrations in Aerva lanata was studied using SEM-EDX technique. The aim of this study is to determine qualitatively and quantitatively trace elements in Aerva lanata and their medicinal roles in the hu...
COMPLEMENTARY AND ALTERNATIVE MEDICINE (CAM) THERAPIES FOR MANAGEMENT OF PAIN RELATED TO ENDOMETRIOSIS
Endometriosis is a gynaecological disease with a complex etiology. It is associated with severe pelvic pain, sub fertility and reduced quality of life. Endometriosis has a multifactorial etiology and therefore its manage...
FORMULATION AND EVALUATION OF SUSTAINED RELEASE PELLETS OF TRAMADOL HYDROCHLORIDE
The aim of the present research is to develop and evaluate a better sustained release multiple unit pellets (MUP) formulation of Tramadol hydrochloride. Dissolution and diffusion controlled systems have classically been...