SPECTROSCOPIC ANALYSIS AND ANTIBACTERIAL EFFICACY OF BIOACTIVE COMPOUNDS FROM LIMONIA ACIDISSIMA L. FRUIT EXTRACT AGAINST CLINICAL PATHOGENS
Journal Title: International Journal of Pharmacy and Pharmaceutical Sciences - Year 2015, Vol 7, Issue 3
Abstract
Objective: To determine the phytochemical composition using spectral analysis and antibacterial activity of Limonia acidissima fruit methanol extract.Methods: Phytochemical analysis was performed to determine the total alkaloids, saponins, total phenols and flavonoids content of fruit extract. Secondary metabolite compounds were determined performed by FT-IR and GC-MS analysis. Antibacterial activity assay was performed using agar well diffusion technique.Results: The alkaloid and saponins contents of the crude methanol extract were found to be 38.47 g/100 g and 0.13 g/100 g dry matter respectively. The LF methanol extract showed total phenol contents of 33.38 µg/mg and the flavonoids was found to be 33.80 µg/mg extract. FT-IR analysis indicated the presence of phenols, alkanes, amino acids, α, β-unsaturated esters, alkenes, nitro compounds, aromatics, aliphatic amines, carboxylic acid, alkenes, and alkyl halides functional groups. The GC-MS analysis revealed the presence of linoleic acid, octadecanoic acid, hexadecanoic acid, maltol, vinly guaiacol, furanone, and ascorbic acid. The antibacterial activity assay showed dose dependent inhibition against clinical pathogens.Conclusion: This study elucidated the prospective value of L. acidissima fruit as a nutritional and medicinal source. Â
Authors and Affiliations
Srinivasan Ponnuraj, Dineshbabu Jaganathan, Manimekalai Kanagarajan, Teepica Priya Darsini Deivamarudachalam
Keywords
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