STABILITY INDICATING ANALYTICAL METHOD DEVELOPMENT AND VALIDATION OF MEMANTINE HCl AND DONEPEZIL HCl USING RP-HPLC
Journal Title: International Journal of Pharmacy and Pharmaceutical Sciences - Year 2015, Vol 7, Issue 11
Abstract
Objective: To develop and validate stability indicating method for the analysis of Memantine HCl and Donepezil HCl.Methods: The chromatographic separation was performed on Hypersil BDS (4.6 x 150 mm, 5m) using Sodium dihydrogen ortho phosphate: Acetonitrile (30:70v/v) at a flow rate of 1 ml/min and detection of both the eluents was carried out by UV Detector.Results: The Retention time of Memantine HCl and Donepezil HCl were found to be 2.833 min. and 4.777 min respectively. Method was found to be linear over the range of 40-120μg/ml for Memantine HCl and 20-60μg/ml for Donepezil HCl. Percentage recovery of Memantine HCl and Donepezil HCl was found to be 99.62% and 99.45% respectively. The percentage purity thus found is 98.5% and 98.6% for Memantine HCl and Donepezil HCl. The Limit of Detection of Memantine HCl and Donepezil HCl is 3.69 mg/ml and 2.72 mg/ml. and Limit of Quantification of Memantine HCl and Donepezil HCl is 11.13 mg/ml and 8.25 mg/ml respectively. In the stress degradation studies, it was found that Memantine HCl and Donepezil HCl showed no degradation in acid (0.1M HCl), base (0.1M NaOH), peroxide, heat and sunlight.Conclusion: A new sensitive, simple, and stability indicating high performance layer chromatographic (HPLC) method has been developed and validated for determination of Memantine HCl and Donepezil HCl. The proposed method can be used for routine determination of Memantine HCl and Donepezil HCl stability.Â
Authors and Affiliations
Syeda Noorain Amena, S. H. Rizwan
Keywords
Related Articles
- EP ID EP578154
- DOI -
- Views 87
- Downloads 0
ENTADA PHASEOLOIDES ATTENUATES SCOPOLAMINE INDUCED MEMORY IMPAIRMENT, NEURO-INFLAMMATION AND NEURO-DEGENERATION VIA BDNF/TRKB/NFΚB P65 PATHWAY IN RADIAL ARM MAZE
Objective: The present study investigates the protective effects of crude extract from seeds of Entada phaseoloides and isolated compounds in enhancing cognition in Scopolamine induced learning and memory impairments in...
IN VITRO PHOTO-TOXICITY SCREENING OF HYDROALCOHOLIC EXTRACT OBTAINED FROM MANILKARA ZAPOTA (L.) ROYEN LEAVES
Objective: Cosmetic products like sunscreens are being used widely nowadays due to increasing consumer awareness about the harmful effects of ultraviolet light exposure. Conventionally used chemical filters lack photosta...
QUALITATIVE AND QUANTITATIVE DETERMINATION OF SECONDARY METABOLITES AND ANTIOXIDANT POTENTIAL OF FICUS BENGHALENSIS LINN SEED
Objective: To evaluate the phytochemical constituents and the antioxidant activity of ethanolic extract of Ficus benghalensis seed locally prescribed as a diet for peptic ulcer.Methods: Qualitative analysis of various ph...
CLOUD POINT EXTRACTION, PRECONCENRATION AND SPECTROPHOTOMETRIC DETERMINATION OF COBALT IN WATER SAMPLES
Objective: A new cloud point extraction (CPE) method was developed for pre concentration of trace cobalt (II) in water samples.Methods: The method is based on the complexation reaction of Co (II) with 2-(benzothiazolyl a...
OLEOGELS OF OLIVE OIL AND SOYBEAN OIL FOR TOPICAL DRUG DELIVERY: A COMPARATIVE ANALYSIS
Objective: The objective of the present investigation was to develop olive and soybean oil-based oleogels with Span 40 and/or Tween 80 (as gelator and/or surfactant) and determine the critical gelator concentration (CGC)...