Stability Indicating Rp-Hplc Method For The Simultaneous Estimation Of Atazanavir And Cobicistat In Bulk And Tablet Dosage Form
Journal Title: IOSR Journal of Pharmacy and Biological Sciences (IOSR-JPBS) - Year 2017, Vol 12, Issue 5
Abstract
The combination of Atazanavir and Cobicistat combined dosage form is used for the treatment of HIV-1 infection. Review of Literature reveals that there is no stability indicating HPLC methods for simultaneous estimation of atazanavir and cobicistat from tablet dosage form. Hence a High Performance Liquid Chromatographic (HPLC) method was developed and validated for the estimation of Atazanavir and Cobicistat simultaneously. Chromatographic separation was optimized by gradient HPLC on a C18 column [Phenomenex, 250 x 4.6 mm, 5µ] utilizing a mobile phase consisting a mixture of 0.01M sodium acetate buffer of pH 4.2 , methanol and acetontrile in the ratio of 25:15:60 v/v at a flow rate of 1ml/min with UV detection at 235nm. The retention time of Atazanavir and Cobicistat was 5.48 min and 7.02 min respectively. Good Linearity obtained over the range of 25μg/ml to 150μg/ml for atazanavir and cobicistat. Correlation coefficient was found to be 0.999&0.998 for atazanavir & cobicistat respectively. The % RSD of precision for atazanavir and cobicistat was found to be 0.10 and 0.08 respectively. The % mean recovery was found to be 99.19-101.68% for atazanavir and 99.03-99.72.% for cobicistat. The validated economical method was applied for forced degradation study of Atazanavir and Cobicistat tablet.
Authors and Affiliations
S. Naazneen1, A. Sridevi2
Keywords
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- EP ID EP391168
- DOI 10.9790/3008-1205046168
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