Stability of Envelope Proteins of SARS-CoV-2 Variants Could Be a Choice of New Drug Target
Journal Title: International Journal of Experimental Research and Review - Year 2024, Vol 42, Issue 6
Abstract
Severe Acute Respiratory Syndrome Coronavirus-2 (SARS-CoV-2) has emerged as one of the worst viral pandemics during the past few years. As reported by the World Health Organization, around 77,56 49 520 cases and 70 51 720 deaths were reported from all over the world, which includes approximately 3500 patients reported during the recent past only. Currently, circulating variants of SARS-CoV-2 are KP.3, JN.1, BA.2.86 and KP.2. Mass vaccinations have been provided since the end of December 2020, which led to 5.47 billion people vaccinated till date. However, the disease continues in small foci all over the world. Development of an effective drug target and mutation independent vaccine thus becomes essential research priorities. Owing to the unavailability of a specific drug molecule, the present study has focused on the development of an effective drug target to treat COVID-19. In-house primers were designed for four essential structural genes viz., Spike protein, ORF1ab, Nucleocapsid gene, and Envelope gene. Samples of different waves were amplified using these primers employing the Polymerase Chain Reaction (PCR) assay. A total number of 86 SARS-CoV-2 RT PCR positive samples were studied, and results showed the most frequent appearance (80.2%) of Envelope (E) protein in all the samples. This suggests that during transmission across numbers of human hosts, it is the Envelope protein that was the most stable one. The most severe Delta variant showed the presence of E proteins in all the samples assayed. Blocking E protein as a new drug target to intervene intracellular replication of virus could be an effective drug development strategy.
Authors and Affiliations
Ambreen Shafaat Khan, Bennet Angel, Annette Angel, Vinod Joshi, Shareef BM, Poorna Khaneja, Bhawna Sharma, Nuzhat Maqbool Peer, Neha Singh, Shilpa Barthwal, Satendra Pal Singh, Ramesh Joshi, Rajesh Thakur, Monika Dheer, KhushbuKumari, Aarya Chitransh, Reshu Chauhan, Lalmalsawmi Sailo
Keywords
Related Articles
- EP ID EP743739
- DOI 10.52756/ijerr.2024.v42.010
- Views 27
- Downloads 0
Monkeypox Detection Using Hyper-Parameter Tuned Based Transferable CNN Model
The reemergence of Monkeypox, a communicable illness resulting from the Monkeypox virus, has raised apprehensions about a potential swift global pandemic similar to the COVID-19 epidemic as COVID-19 infections diminish g...
Popping balls papaya extract: Preparation of pediatric dosages in therapeutic formulations for therapeutic usage in dengue and malaria
Spherification is a cutting-edge molecular gastronomy method that has just emerged in the realm of food science and technology, and it may be used to produce foods with superior sensory qualities and a high level of cons...
Management of root-knot nematodes, Meloidogyne incognita in Okra using wheat flour as bionematocides
The current study aims to determine how well root-knot nematodes (Meloidogyne sp.) are reduced when wheat flour is used as bio-nematicides to increase agricultural productivity. Root-knot nematodes inflict a significant...
Enhancing Academic Integrity: An Analysis of Advanced Techniques for Plagiarism Detection using LESK, Word Sense Disambiguation, and SVM
Plagiarism is widespread in academia, from ancient literature to modern research, where scholars' work is copied and published without authorization. In the late 90s, researchers explored various methods to detect plagia...
Empowering rural women by participating in sustainable environmental management: A case study of Banasthali University, Rajasthan
In this paper, an attempt has been made to examine the rural women living around the Banasthali University, Rajasthan and how they empowered themselves by participating in sustainable environmental management programmes...