Stable Isotope Dilution Mass Spectrometry for Membrane Transporter Quantitation
Journal Title: The AAPS Journal - Year 2013, Vol 15, Issue 4
Abstract
This review provides an introduction to stable isotope dilution mass spectrometry (MS) and its emerging applications in the analysis of membrane transporter proteins. Various approaches and application examples, for the generation and use of quantitation reference standards—either stable isotope-labeled peptides or proteins—are discussed as they apply to the MS quantitation of membrane proteins. Technological considerations for the sample preparation of membrane transporter proteins are also presented.
Authors and Affiliations
Vahid Farrokhi, Adam J. McShane, Reza Nemati, Xudong Yao
Keywords
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- EP ID EP681182
- DOI 10.1208/s12248-013-9529-8
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