STAINING OF HISTOLOGICAL SECTIONS FROM THE SMALL INTESTINE USING HIBISCUS SABDARIFFA
Journal Title: Journal of Biomedical and Pharmaceutical Research - Year 2014, Vol 3, Issue 5
Abstract
Objective: this was a descriptive study aimed to assess the quality of staining of intestinal tissue by Hibiscus Sabdariffa solution compared to Hematoxylin-Eosin routine stain. Methods: paraffin-embedded formalin-fixed tissue sections from small intestine were stained by Hibiscus Sabdariffa solution using different concentrations and time durations in room temperature. Results: With 5 % solution, 48 % of slides showed at least very good quality of staining. The excellent results were mainly obtained when time duration was 60 minutes while poorest results were obtained when staining time was 1-2 minutes Conclusion: Hibiscus Sabdariffa solution is a cheap, natural, and harmless dye that can be used for staining of histological sections and it is comparable to the routine Hematoxylin and Eosin stain.
Authors and Affiliations
Abd-Alhafeez Ibnouf*| Department of Medical Laboratories, Portsudan Ahlia College, Portsudan, Sudan., Khalid Adam| Department of Molecular Biology, Faculty of Laboratory Medical Science, Neelain University, Khartoum, Sudan., Esam AbdulRaheem| Department of Medical Laboratories, College of Applied Medical Science, Shaqra University, Saudi Arabia., Ali Ageep| Department of Pathology, Red Sea University, Portsudan, Sudan.
Keywords
Related Articles
- EP ID EP3411
- DOI -
- Views 392
- Downloads 18
GENOTYPIC AND BIOCHEMICAL CHARACTERIZATION OF PASTEURELLA MULTOCIDA ISOLATED FROM SHEEP AT SLAUGHTER IN AMMAN-JORDAN
Pasteurella multocida (p.m.) is a gram negative coccobacilli bacterial pathogen, classified into three subspecies, five capsular serogroups, which is the causative agent of a range of diseases in domestic animals of econ...
DETECTION OF GENETIC POLYMORPHISM OF NAD (P) H: QUINONE OXIDOREDUCTASE 1 (NQO1) AMONG SUDANESE PATIENTS WITH ACUTE MYELOID LEUKAEMIA
AML is a prevalent haematological cancer among Sudanese population. Previous studies reported an association between NQO1 polymorphism and leukaemia, however these studies showed differences in the occurrence and frequ...
DETECTION OF EXTENDED SPECTRUM BETA - LACTAMASE PRODUCING E. COLI & KLEBSIELLA SPECIES CAUSING URINARY TRACT INFECTION
Extended-spectrum beta-lactamases (ESBLs) constitute a growing class of plasmid-mediated ß-lactamases which confer resistance to broad spectrum beta-lactam antibiotics. The frequency of ESBL producing strains among cli...
Application of omics in personalized oral health care: A paradigm shift
Periodontal disease and Dental caries are the most common oral diseases in adult populations worldwide and is a major public health concern due to its substantial cost to the oral health care system. During recent years...
Serofrecquancy of Human Immuno Deficiency virus among Known hepatitis Bvirus infected patients attending Talodihospital, SouthkordofanState, Sudan
Background: Co infection with human immuno deficiency virus (HIV) and hepatitis B virus (HBV) is common; worldwide, an estimated 10% of HIV-infected persons have chronic hepatitis B; because The incidence of traditional...