State of Chimerism as a Promising Tool for Follow up of Hematopoietic Stem Cell Transplantation in Egyptian Patients
Journal Title: Journal of Medical Science And clinical Research - Year 2016, Vol 4, Issue 7
Abstract
Follow up of patients after HSCT by chimerism detection can detect the outcome either engraftment, relapse or rejection. Many variables affect the result of chimerism either increasing or decreasing chimerism. Lineage specific chimerism is specific and sensitive than chimerism detection by whole blood. Here in this study the importance of these variables on the outcome of chimerism detection is studied. Subject and method: This study was conducted at Ain Shams University Hospital during the periodbetween January 2011and January 2015. 46 patients were included (16 child and 30 adult). They weretransplanted for malignant and non malignant hematological diseases from totally matched siblings.Themedian duration of follow-up was one year. Chimerism status was detected for all patients by polymerase chain reaction based on amplification of variable number tandem repeat (VNTR) markers. Six VNTR loci were detected in every subject pre-transplant in order to detect an informative locus to be used in follow uppost-transplant. Also lineage specific chimerism and dilution experiment were conducted on some patients. Results: One patient showed no informative locus pre-transplantation, 37 patients showed complete donor chimersm, two patients retained recipient pattern post-transplantation and six patients showed MC. Lineage cell specific chimerism and dilution experiment were conducted for patients who showed failure or mixed chimerism. Conclusion: Chimerism detection by VNTR is dependable method to follow up patients after BMT however,some cases need some modifications as repeated analysis, short duration, lineage specific in comparison to whole blood analysis, dilution experiments in order to predict the outcome
Authors and Affiliations
Hala A Talkhan
Keywords
Related Articles
- EP ID EP213825
- DOI -
- Views 81
- Downloads 0
A Clinical Study and Follow Up of Blunt Injury Abdomen in the Department of General Surgery
Objectives: 1) To know the etiology and incidence. 2) To study the nature and incidence of injury to different intra abdominal organs. 3) To assess the importance of the various investigations. 4) To study the post opera...
Treatment Modalities for Basal Like Breast Cancer
Breast cancer represents a heterogeneous group of disorders with significant morbidity and mortality among female population. Basal like breast cancer represents one of the several sub types of breast cancer. It has alwa...
Bile Duct Injuries Following Laparoscopic Cholecystectomy and their Outcome
Introduction: Bile duct injuries are still among the most common and dreadful complications, following laparoscopic cholecystectomies despite advancement of training and technology. Methods: Prospective study of bile duc...
Getting Old Age Home Residents out of Depression: Effect of a Physical Exercise Program
Depression is often referred as the “common cold” of mental illness. Prevalence of depression in elderly is high worldwide. In India it contributes to a substantial burden. Treatment of depression is inadequate for older...
A comparative evaluation of three different methods used to achieve local anaesthesia for fiberoptic guided intubation under sedation
Background and Aim: Fiberoptic intubation is the gold standard technique for airway management in patients with difficult standard direct laryngoscopy, performed either awake or under sedation. The aim of sedation is to...