State photosynthetic apparatus of rose essential oil after adaptation to conditions in vivo
Journal Title: Naukovyi visnyk Chernivetskoho universytetu. Biolohiia (Biolohichni systemy) - Year 2017, Vol 9, Issue 2
Abstract
For effective adaptation clones of rose essential oil grade Lan was chosen optimal conditions in vivo and the type of substrate. The best way to adapt and grow plants, before laying plantations, is to adapt in greenhouse conditions using a mixture of peat and perlite (2:1), which promotes the rapid elongation of the clones. Regenerates with a height of less than 3.5 cm are optimal for transferring to in vivo conditions. The most successful period of plants transplantation from in vitro to in vivo conditions is the end of May - the beginning of June, and from in vivo to ex vitro conditions the end of July - the beginning of August. Electromagnetic absorption spectra of energy by plastidic pigments show that plants in the conditions of open soil of roses essential oils increase the ability to absorb light in 2,0-2,3 times. The main maxima of absorption of electromagnetic energy do not change. The ratio of chlorophylls to the adaptation period is proportional to the increase. When the plants are transferred to small plots, after the initial adaptation, the amount of chlorophylls decreases. The leaves of plants intensified the synthesis of the protective group of pigments, due to new conditions of growth. This caused a decrease in the ratio of the sum of chlorophylls to carotenoids. Linear growth in the leaves of the total amount of phenolic compounds and phenolic antioxidants indicates the mobility of their system. The number of total phenols increased by 6 times, and catechins and phenolic anti-oxidants by 7 times over the entire period of adaptation. As a consequence, it is appropriate to use vanillin acid as a phenylpropanoid synthesis activator to accelerate the process of accumulation of protective metabolites. Thus, monitoring of the state, regenerates of plants during their adaptation by biochemical markers allows optimization of the control regime of plants, with their varietal specific properties. Development of reference methods for controlling the state of plants: by pigment composition, quantitative and qualitative indicators of secondary metabolism products using fluoroscence measurement methods of chlorophylls - allows to create soratoadaptation systems.
Authors and Affiliations
О. О. ОЛІЙНИК, М. Д. МЕЛЬНИЧУК
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