Statistical analysis of cellulase production in Bacillus amyloliquefaciens UNPDV-22
Journal Title: Extreme Life, Biospeology & Astrobiology - International Journal of the Bioflux Society - Year 2011, Vol 3, Issue 1
Abstract
The production of cellulase in Bacillus amyloliquefaciens UNPDV-22 was optimized using response surface methodology (RSM). Central composite design (CCD) was used to study the interactive effect of fermentation medium components (wheat bran, soybean meal, and malt dextrin) on cellulase activity. Results suggested that wheat bran, soybean meal, and malt dextrin all have significant impact on cellulase production. The use of RSM resulted in a 70% increase in the cellulase activity over the control of non-optimized basal medium. Optimum cellulase production of 11.23 U/mL was obtained in a fermentation medium containing wheat bran (1.03%, w/v), soybean meal (2.43%, w/v), and malt dextrin (2.95%, w/v).
Authors and Affiliations
Vasudeo Zambare, Lew Christopher
Keywords
Related Articles
- EP ID EP92359
- DOI -
- Views 192
- Downloads 0
Statistical analysis of cellulase production in Bacillus amyloliquefaciens UNPDV-22
The production of cellulase in Bacillus amyloliquefaciens UNPDV-22 was optimized using response surface methodology (RSM). Central composite design (CCD) was used to study the interactive effect of fermentation medium c...
Extremophiles and extremozymes: Importance in current biotechnology
The use of biocatalysts in different biochemical reactions is generally restricted to mild ambient conditions. However, the enzymes produced by different living organisms inhabiting ecosystems with extreme conditions of...
A short review on tardigrades – some lesser known taxa of polyextremophilic invertebrates
Tardigrades are polyextremophilic small organisms capable to survive in a variety of extreme conditions. By reversibly suspending their metabolism (cryptobiosis – tun state) tardigrades can dry or freeze and, thus, survi...
Methane in outer space: The limit between organic and inorganic
Methane is the principal component of natural gas, the simplest alkane, and among the most abundant organic compounds on our planet; it is also found in outer space in enormous amounts. The extremely abundant and natural...
Plantago cornuti Gouan, a rare halophyte: sites from Transylvanian Depression (Romania)
This paper is a brief summary on the rare halophile species Plantago cornuti Gouan’s populations in the Transylvanian Depression (Romania). In this work were listed and characterized a number of sites across the Transyl...