Statistical analysis of cellulase production in Bacillus amyloliquefaciens UNPDV-22
Journal Title: Extreme Life, Biospeology & Astrobiology - International Journal of the Bioflux Society - Year 2011, Vol 3, Issue 1
Abstract
The production of cellulase in Bacillus amyloliquefaciens UNPDV-22 was optimized using response surface methodology (RSM). Central composite design (CCD) was used to study the interactive effect of fermentation medium components (wheat bran, soybean meal, and malt dextrin) on cellulase activity. Results suggested that wheat bran, soybean meal, and malt dextrin all have significant impact on cellulase production. The use of RSM resulted in a 70% increase in the cellulase activity over the control of non-optimized basal medium. Optimum cellulase production of 11.23 U/mL was obtained in a fermentation medium containing wheat bran (1.03%, w/v), soybean meal (2.43%, w/v), and malt dextrin (2.95%, w/v).
Authors and Affiliations
Vasudeo Zambare, Lew Christopher
Keywords
Related Articles
- EP ID EP92359
- DOI -
- Views 162
- Downloads 0
Extreme ambiences vis-à-vis endogenous antioxidants of Marwari goat from arid tracts in India
To investigate the ambience associated variations in endogenous antioxidants status of Marwari goat of arid tracts in India, blood samples were collected to harvest sera from male and female Marwari goats of varying age...
Twig extract of the apple mangrove affects the activities of trypsin, chymotrypsin and lipase in postlarval black tiger shrimp Penaeus monodon at varying feeding frequencies
This study aimed to determine the effect of the twig extract of the apple mangrove Sonneratia caseolaris on the activities of trypsin, chymotrypsin and lipase in the postlarval black tiger shrimp Penaeus monodon. Incorpo...
Enzymological characterization of the sapropelic muds in the lakes Ocna Sibiului.
Besides purely scientific interest, the spa waters and therapeutic muds consecrated research have a remarkable practical importance. The evaluation of the water and therapeutical muds’s physicochemical, microbiological a...
Heavy metals effect on the E. coli biofilms isolated from the polluted waters of Arieş river (Romania)
Objective: Biofilms are surface-attached microbial communities consisted by multiple layers of cells embedded in a hydrated matrix. Biofilms are dynamic microbial communities in which transitions between planktonic and s...
The influence of Plant Growth Promoting Rhizobacteria (PGPR) on the reduction of abiotic stresses in crops
Plants are always subjected to biotic and abiotic stresses in the environment which have influences on the growth and development of the plants. Beneficial free-living soil bacteria are usually referred as Plant-Growth...