Statistical analysis of cellulase production in Bacillus amyloliquefaciens UNPDV-22
Journal Title: Extreme Life, Biospeology & Astrobiology - International Journal of the Bioflux Society - Year 2011, Vol 3, Issue 1
Abstract
The production of cellulase in Bacillus amyloliquefaciens UNPDV-22 was optimized using response surface methodology (RSM). Central composite design (CCD) was used to study the interactive effect of fermentation medium components (wheat bran, soybean meal, and malt dextrin) on cellulase activity. Results suggested that wheat bran, soybean meal, and malt dextrin all have significant impact on cellulase production. The use of RSM resulted in a 70% increase in the cellulase activity over the control of non-optimized basal medium. Optimum cellulase production of 11.23 U/mL was obtained in a fermentation medium containing wheat bran (1.03%, w/v), soybean meal (2.43%, w/v), and malt dextrin (2.95%, w/v).
Authors and Affiliations
Vasudeo Zambare, Lew Christopher
Keywords
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