Storage artefacts in peripheral blood smears
Journal Title: IP Journal of Diagnostic Pathology and Oncology - Year 2018, Vol 3, Issue 3
Abstract
Introduction Microscopic evaluation of peripheral blood smear is a very important investigation Blood for various laboratory analyses are commonly kept in ethylene diamine tetra acetic acid EDTA EDTA can however cause morphological and fragility changes in blood cells However morphological analyses can be greatly hampered due to occurrence of artefactsAims and Objectives To identify the storage artefacts and thus avoid misinterpretation of peripheral blood smearsMaterials and Methods 50 blood samples were obtained using EDTA and 35 sodium citrate as anticoagulants Smears were made immediately as well as after 2 4 and 6hrs of storage at room temperature Smears obtained from same patients by finger prick method served as controlsConclusion EDTA has been recommended as the anticoagulant of choice for peripheral smear but should be examined within 1hr of collection to avoid misinterpretation as pathological findings leading to wrong diagnosisKeywords Peripheral smears Anticoagulant Platelets Neutrophils Artifacts
Authors and Affiliations
Shaista Choudhary, Ravi Shankar Katkar, Divya Nagaram
Keywords
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- EP ID EP482764
- DOI 10.18231/2581-3706.2018.0039
- Views 99
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