Storage artefacts in peripheral blood smears
Journal Title: IP Journal of Diagnostic Pathology and Oncology - Year 2018, Vol 3, Issue 3
Abstract
Introduction Microscopic evaluation of peripheral blood smear is a very important investigation Blood for various laboratory analyses are commonly kept in ethylene diamine tetra acetic acid EDTA EDTA can however cause morphological and fragility changes in blood cells However morphological analyses can be greatly hampered due to occurrence of artefactsAims and Objectives To identify the storage artefacts and thus avoid misinterpretation of peripheral blood smearsMaterials and Methods 50 blood samples were obtained using EDTA and 35 sodium citrate as anticoagulants Smears were made immediately as well as after 2 4 and 6hrs of storage at room temperature Smears obtained from same patients by finger prick method served as controlsConclusion EDTA has been recommended as the anticoagulant of choice for peripheral smear but should be examined within 1hr of collection to avoid misinterpretation as pathological findings leading to wrong diagnosisKeywords Peripheral smears Anticoagulant Platelets Neutrophils Artifacts
Authors and Affiliations
Shaista Choudhary, Ravi Shankar Katkar, Divya Nagaram
Spectrum of histopathological evaluation of neoplastic lesions of gastrointestinal tract- A 3 years retrospective study
Introduction Gastrointestinal tract is the common site of cancer in both men women throughout the world Gastrointestinal cancer causes high mortality morbidity Diagnosis of these lesions requires endoscopy followed by b...
Perplexing Multifocal Abdominopelvic Mass - turned out to be actinomycosis
A 34 year old female presented with complaints of lower abdominal pain and abnormal uterine bleeding for 2 months. On examination subcutaneous swelling on anterior abdominal wall and bilateral pelvic adnexal mass was not...
Pilomatrix carcinoma - pilomatrixoma: Two ends of the disease spectrum
Pilomatrixoma pilomatrix carcinoma are neoplasms of skin appendage with differentiation towards hair follicle matrix cells They represent two ends of the spectrum with varying clinical and pathological characteristics Pi...
Nodular lymphoid hyperplasia of lung masquerading as tuberculosis
Nodular lymphoid hyperplasia (NLH) of the lung is a rare entity that is considered to be a benign reactive lymphoid hyperplasia. It shows localised reactive polyclonal lymphoid proliferations. Due to scant literature, ve...
White sponge nevus- A rare entity
White sponge nevus (WSN) is an autosomaldominantly inherited form of mucosal leukokeratosis. It was first described by Hyde in 1909 and named by Cannon in 1935. Hence, it is also called Cannon's disease, hereditary leuko...