Structural changes of loach embryos during embryogenesis under the influence of Flurenizyd
Journal Title: Journal of Animal Science, Biology and Bioeconomy - Year 2016, Vol 34, Issue 4
Abstract
With the aim of exposing the harmful influence of Flurenizyd, the studies investigated the ultrastructure and morphology of embryos of loach Misgurnus fossilis L. in early stages of development and larval development on the first days of incubation environment, which contained this antibiotic.. It was shown that the antibiotic at the concentrations of 1 mМ, 5 mМ, 15 mМ caused a high death rate of embryos of loach already on the first day of its application. It was established that Flurenizyd caused structural disturbances of the mitochondria and the cytoplasmic net, in addition to causing an increase of the number of lysosomes in the embryos of loach at the stage of development of 2 blastomeres. The degree of these changes is dose dependent. Flurenizyd at the concentrations of 0.05 mМ, 0.15 mМ and 5 mМ at the stage of development of 64 blastomeres leads to dystrophic changes. It was shown that the antibiotic at the concentration of 0.05 mМ and 0.15 mМ causes necrotic changes, while at the maximal investigated concentration the influence of Flurenizyd on the structural changes of blastomeres is less visible.
Authors and Affiliations
NATALIYA OLEKSANDRIVNA BODNARCHUK, OLEKSANDR ROMANOVYCH KULACHKOVSKY, SVITLANA MYKHAYLIVNA MANDZYNETS, DMYTRO IVANOVYCH SANAGURSKIY
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