Structure of Single Particles from Randomly Oriented Ensembles Using an X-Ray Free Electron Laser
Journal Title: Biomedical Journal of Scientific & Technical Research (BJSTR) - Year 2017, Vol 1, Issue 7
Abstract
The method of time-resolved crystallography offers a method of studying fast changes in structure during a chemical reaction. However, being a crystallographic method it is restricted to crystal structures. The problem with this is that most chemical reactions under physiological conditions happen in solution and not on molecules which are part of a crystal. The main problem with trying to find chemical reactions in solution is that one needs a powerful source of X-rays which will give a measurable signal even from small numbers of randomly oriented molecules. The newly developed X-ray free electron laser allows this when combined with a novel theoretical technique. Of course we understand one of the strengths of working with crystals is that the signal comes not from one but from trillions of identically oriented unit cells. The fact that the unit cells are identically oriented means that the X-rays do not have to scatter off a single molecule (or unit cell) to give a sensible signal. Since all molecules are identically oriented information about an idealized average molecule may be obtained from scattering by a crystal even though the different photons scatter off different molecules. The problem is that most chemical reactions do not take place in crystals.
Authors and Affiliations
SS Kim, DK Saldin, S Wibowo
Keywords
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- EP ID EP572667
- DOI 10.26717/BJSTR.MS.ID.000574
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