STUDIES ON ACUTE TOXICITY OF HEPTOPLUS IN EXPERIMENTAL RATS
Journal Title: International Journal of Pharmacy and Pharmaceutical Sciences - Year 2016, Vol 8, Issue 11
Abstract
Objective: To evaluate acute toxicity of Heptoplus (polyherbal drug) in Sprague-Dawley rats and to identify the active ingredients of the herbal drug.Methods: Heptoplus was subjected to preliminary phytochemical screening and the HPTLC fingerprint profile of herbal drug was documented. OECD guideline 423-Acute toxic class method was followed to evaluate the oral toxicity of Heptoplus in Sprague-Dawley rats. In acute toxicity studies, Group I (control) rats received 0.5% of carboxymethyl cellouse (Vehicle). Group II rats received 2000 mg/kg b. w of Heptoplus. The rats were observed on the day of dosing and thereafter for 14 d, for any toxic effect. Results: Preliminary phytochemical analysis of Heptoplus revealed total phenol, flavonoid, carbohydrate, and tannins as its major constituents. The total phenol and flavonoid content of Heptoplus was found to be 170 μg of gallic acid and 162 μg of quercetin equivalent. HPTLC analysis proved that phyllanthin is an active compound of Heptoplus. Acute oral toxicity assays showed Heptoplus administration did not result in any treatment-related mortality, abnormal clinical signs, and loss of body weight or gross pathological changes in rats. Hence, LD50 value of Heptoplus was found to be greater than 2000 mg/kg b. wt.Conclusion: Heptoplus contain phyllanthin as an active ingredient. LD50 value of Heptoplus was found to be greater than 2000 mg/kg b. wt.
Authors and Affiliations
M. Sankar, Johanna Rajkumar
Keywords
Related Articles
- EP ID EP575895
- DOI 10.22159/ijpps.2016v8i11.13934
- Views 60
- Downloads 0
HISTONE ACETYLTRANSFERASE P300/CBP-ASSOCIATED FACTOR INHIBITION BY QUERCETIN AS ANTICANCER DRUG CANDIDATE WITH IN SILICO AND IN VITRO APPROACH
Objective: The objective of this research was to show quercetin potency to inhibit histone acetyltransferase p300/CBP-associated factor (HAT PCAF) activity. Molecular docking study was used to show inhibition model of qu...
STROKE OUTCOMES IN NON-DIABETIC, DENOVO DIABETIC AND DIABETIC INDIAN PATIENTS MEASURED BY MODIFIED RANKIN SCALE: AN OBSERVATIONAL STUDY
Objective: Hyperglycemia is a known risk factor which adversely impacts the outcomes in stroke patients compared to patients with normal blood glucose levels. Patients suffering from an acute stroke who are previously no...
EVALUATION OF THE ANTIFUNGAL ACTIVITIES OF THREE ESSENTIAL OIL COMPONENTS AGAINST PENICILLIUM EXPANSUM SPORES
Objective: The aim of the present study was to evaluate the antifungal potential of β-Ionone; carvone and 1,8-cineole which are three essential oil components.Methods: For that purpose, the minimum inhibitory (MIC) and...
SYNTHESIS, DOCKING STUDIES AND EVALUATION OF ANTIMICROBIAL AND IN VITRO ANTIPROLIFERATIVE ACTIVITY OF 5H-CHROMENO 4,3-D PYRIMIDIN-2-AMINE DERIVATIVES
Objective: Docking studies and synthesis of 4-aryl-5H-chromeno [4,3 -d]pyrimidine-2-amine derivatives to evaluate antimicrobial and in vitro cytotoxicity activity.Methods: Docking studies were performed on Autodock Vina....
THE EFFECT OF METFORMIN ON NON-ENZYMATIC GLYCOSYLATION OF RECOMBINANT HUMAN SERUM ALBUMIN
Objective: The present research work was aimed to observe the effect of Metformin hydrochloride (MET) on non-enzymatic glucosylation of recombinant human serum albumin (rHSA).Methods: Albumin was incubated at 37 ° in 10...