Abstract

The study evaluates the antimicrobial potential of methanol extract of Date palm bark and to identify potential natural sources for the synthesis of new drugs to address the alarming proposition of growing antimicrobial resistance over the course of last three decades. The crude methanol extract of Bark of Date palm, Phoenix dactylifera was extracted and antibacterial activity was evaluated by paper disc method showed enterprising potential zone of inhibition of 17 mm against S.typhi and K.pneumoniae leading to the further investigation using agar cup method on various standard strains demonstrated 22mm zone of inhibition by Shigella. MIC of E.coli found in the range of 3.12 mg/ml and 0.78mg/ml for S.aureus. The MBC for E.coli of Bark crude methanol extract showed 1.25mg/ml and for S.aureus is 1.5mg/ml. Bark Methanol fraction exhibited excellent activity against AST of Activity guided fraction with a zone of inhibition of 20 mm against E.coli and 22mm against S.aureus. Phytochemical analysis reveals the presence of glycoside, saponins, alkaloids, flavonoids and tannins. Characterization of bioactive compounds from the potent bark methanol fraction analysed by HPTLC, followed by bioautography, CHNS, FTIR, LCMS and GCMS analysis profiling the presence of Lup-20(29)-en-3-ol-acetate,(3α) may the bioactive compound reporting antibacterial, antiviral and antioxidant activity. The present study also reveals the potential antioxidant activity (12.08mg/ml), MIC of antiviral activity is 780μg/ml and antiviral activity is 99.29 % against tested coli phage. The phage inactivation kinetics indicates 70% inactivation at 20min of exposure. The methanol bark fraction showed a strong ability to inhibit the infectivity of coli phage and completely prevented bacterial lysis, which it is hoped will promote research into its potential as a novel antiviral agent against pathogenic human viruses. Bark methanol fraction showed an excellent antibacterial activity ananist MDR pathogens in the range 12-14 mm of zone of inhibition revealing its potential as defence tool against array of microbes.

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