Studies on Bacterial Synthesis of Silver Nanoparticles and its Synergistic Antibacterial effect with antibiotics against Selected MDR Enteric Bacteria
Journal Title: SSR Institute of International Journal of Life Sciences (SSR-IIJLS) - Year 2018, Vol 4, Issue 4
Abstract
In the present study, the extracellular synthesis of silver nanoparticles was done using two different bacterial strains viz. Bacillus flexus and Bacillus pseudomycoides. The silver nanoparticles were confirmed by in color test and characterized by UV-visible spectroscopy and the λmax observed at 430 nm and 410 nm confirmed to the synthesis of AgNPs. FTIR analysis confirmed the presence of elemental silver and reveals the dual function of the biological molecule responsible for the reduction and stabilization of AgNPs in the aqueous medium. The XRD showed that silver nanoparticles produced crystalline in nature with size ranges from 30 to 70 nm. The SEM showed that produced silver nanoparticles were spherical, pseudo-spherical in shape with traces of agglomeration. Further through investigation of antibiotic sensitivity/resistant pattern expressed that out of eighteen virulent enteric bacterial isolates, three isolates showed MAR index equal to 1, which indicated the presence of multiple drug resistance (MDR). MIC values of AgNPs against MDR isolate E7 and K3 were established to be 80 μg/ml whereas, for isolate Sa1 the MIC value was 70 μg/ml. The synergistic effect of antibiotics in conjugation with biologically synthesized AgNPs encourages the susceptibility amongst the tested bacterial cultures; viz. Salmonella followed by Klebsiella and Escherichia coli.
Authors and Affiliations
Payal Agrawal, Nikhilesh Kulkarni
Keywords
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- EP ID EP671021
- DOI 10.21276/ijlssr.2018.4.4.7
- Views 96
- Downloads 0
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